mashandiro anoita centrifuge mukuparadzanisa

Mashandiro anoita Centrifuge mukuparadzana

Nhanganyaya yeCentrifugation uye Kukosha Kwayo Mumaitiro Ekuparadzanisa

Kubatanidza zvinhu (centrifugation) inzira inokosha inoshandiswa muzvikamu zvakasiyana-siyana zvesainzi, kusanganisira chemistry, biology, uye mushonga, pakuparadzanisa zvidimbu zvakasimba kana musanganiswa wemvura-mvura. Iyi nzira ine simba uye inoshanda inoshandisa misimboti yesimba repakati kuti tiwane kupatsanurana. Muchinyorwa chino, tichaongorora zvakadzama mashandiro ari shure kwe centrifuge uye toongorora kukosha kwayo mumaitiro ekupatsanurana.

Kunzwisisa Magadzirirwo neZvikamu zveCentrifuge

Centrifuge inowanzova nerotor inofambiswa nemota, chibatiso chemuenzaniso, uye chivharo kana chifukidziro kuti ive nechokwadi chekuti mushandi akachengeteka. Rotor ndiyo chinhu chakakosha chinogadzira simba recentrifugal. Inotenderera nekumhanya kwakanyanya, kazhinji zviuru zvema revolutions paminiti (RPM), ichipa simba rinodiwa kuti pave nekudonha kana kupatsanurwa.

Basa reSimba reCentrifugal mukuparadzana

Simba reCentrifugal isimba rinoonekwa rinoita zvinhu zviri kufamba munzira yakatenderera. Kana musanganiswa ukaiswa mukati me rotor inotenderera, chikamu chakanyanya kusimba mumusanganiswa chinofamba chichienda kumucheto wekunze nekuda kwekusundidzirwa kwekunze kunokonzerwa nesimba reCentrifugal. Panguva iyi, zvikamu zvakareruka zvinofamba zvichienda pakati, zvichigadzira zvikamu zvakasiyana.

Mhando dzeCentrifuges uye Mashandisirwo Adzo

Kune mhando dzakasiyana dzema "centrifuge" dziripo, imwe neimwe yakakodzera maitiro akasiyana ekuparadzanisa uye mhando dzesamples. Dzimwe mhando dzakajairika dzinosanganisira differential centrifuges, preparative centrifuges, ultracentrifuges, uye analytical ultracentrifuges. Differential centrifuges dzinoshandiswa zvakanyanya mumarabhoritari ekurapa ekuisa sedimentation yemasampuli eropa, nepo preparative centrifuges dzichishandiswa kuparadzanisa zvikamu zvakati wandei muhuwandu hwakawanda.

Kune rumwe rutivi, ma "Ultracentrifuge" anogona kukurumidza zvakanyanya uye anoshandiswa muminda yekutsvagisa senge biochemistry ne molecular biology. Michina iyi ine simba guru inogona kuyera musiyano mudiki muhuremu hwema "molecular" uye kubatsira vaongorori kudzidza ma "macromolecules" nemazvo. Ma "analytical ultracentrifuge" ane masisitimu ekuona anogonesa kuongororwa kwe "sedimentation rates" panguva chaiyo, zvichibatsira kudzidza zvakadzama nezvekudyidzana kwema "macromolecules" uye shanduko dzechimiro.

Kufambiswa Kwebasa uye Kuvandudzwa Kwekushanda

Kuti uite kupatsanura uchishandisa centrifuge, muenzaniso unotanga wawedzerwa mumachubhu kana mumidziyo yakakodzera yakagadzirirwa kukwana mukati me rotor. Mushure mekusimbisa chivharo, centrifuge inoiswa kumhanya kwaunoda uye kwenguva yaunoda. Sezvo rotor ichitenderera, zvidimbu zvinorema kana zvikamu zviri mumusanganiswa zvinotanga kugara pasi, zvichigadzira pellet. Panguva imwe chete, zvikamu zvakareruka zvinoumba zvidimbu pamusoro pe pellet zvichienderana nekuwanda kwazvo.

Kuvandudzwa kwekushanda zvakanaka kwakaitwa muma centrifuges emazuva ano kuti pave nekuvandudzwa kwemhedzisiro yekuparadzanisa. Izvi zvinosanganisira magadzirirwo e rotor akasimba uye akachengeteka, masisitimu ekudzora epamusoro ekumhanya kwakaringana nenguva, uye kugona kuita otomatiki maitiro kuti awedzere kubuda, kuderedza zvikanganiso zvevanhu uye kuwedzera kushanda zvakanaka.

Mashandisirwo eCentrifugation muSainzi dzakasiyana

Kuchengetedza masero emasero kunoshandiswa muzvikamu zvakasiyana-siyana zvesainzi. Mukutsvagisa kwezvekurapa, kuparadzanisa DNA, RNA, nemapuroteni kubva musampuli. Kunogonesa kupatsanurwa kwezvikamu zvakasiyana zvemasero, zvakaita semitochondria kana nuclei. Muindasitiri yemishonga, kuparadzanisa masero kunoita basa rakakosha mukuburitsa nekucheneswa kwemakemikari akasiyana-siyana panguva yekugadzirwa kwemishonga.

Musainzi yezvakatipoteredza, centrifugation inobatsira mukubvisa nekuongorora tsvina kubva mumvura nevhu. Iyi inzira yakakosha mukugadzira biofuels, uko inobatsira kuparadzanisa mafuta kana ethanol kubva kubiomass. Pamusoro pezvo, centrifugation inoshandiswa mumaindasitiri ezvekudya nezvinwiwa pakuparadzanisa zvinhu zvakasimba kubva kumvura kana kusiyanisa huwandu hwemvura kuti zvionekwe zvakanaka.

Mukupedzisa, centrifugation inzira ine simba yekuparadzanisa inoshandiswa zvakanyanya muminda yesainzi inoda kupatsanurwa nekucheneswa kwemisanganiswa. Nemhando dzakasiyana dze centrifuges dziri kushandiswa nhasi uye kufambira mberi kuri kuramba kuripo muhunyanzvi, kushanda zvakanaka uye kururama kwekuparadzanisa kuri kuramba kuchifambira mberi, zvichigonesa budiriro mukutsvaga nekugadzira zvinhu zvitsva mumaindasitiri akasiyana-siyana.