ha centrifuge e arohana ho ea holimo

Ha Centrifuge e Arohanngoa ho ea Holimo: Leeto le Hlollang Lefatšeng la Centrifuge

Ho utloisisa Centrifugation le Litšebeliso tsa eona

Saense e Bakang Karohano ea Centrifugal

Mefuta ea Li-Centrifuge le Tšebeliso ea Tsona

Litšebeliso tsa Indasteri le Saense tsa Centrifugation

Likotsi le Meeli ea Karohano ea Centrifugal

Ho utloisisa Centrifugation le Litšebeliso tsa eona

Ho centrifugation ke mokhoa o matla o sebelisoang haholo liindastering tse fapaneng le mafapheng a saense bakeng sa karohano, tlhoekiso le tlhahlobo ea lintho tse fapaneng. Ts'ebetso ena e kenyelletsa tšebeliso ea centrifuge—mochini o hlahisang matla a potolohang ho arola likarolo ka har'a sampole ho latela bongata ba tsona le ho teteana ha tsona. Ka ho beha sampole tlas'a matla a matla a centrifugal, likaroloana tse boima kapa metsoako li lula tlase ho tube kapa setshelo, ha lintho tse bobebe li nyolohela holimo.

Saense e Bakang Karohano ea Centrifugal

Molao-motheo oa saense o ka morao ho karohano ea centrifugal o ho thibela matla a ho phaphamala le a centrifugal. Ha sampole e beoa ka har'a centrifuge, e potoloha ka potlako, e etsa matla a centrifugal a fetang matla a khoheli a sebetsang sampoleng. Sena se fella ka karohano ea likarolo ho latela bongata ba tsona, ka tse teteaneng haholo li eang tlase, li etsa pellet, 'me likarolo tse bobebe li nyolohela holimo, li etsa supernatant.

Ho fihlella karohano e sebetsang hantle, ho bohlokoa ho nahana ka lintlha tse kang khetho ea rotor, lebelo, mocheso le nako. Ka ho fetola lintho tsena tse feto-fetohang, bo-rasaense ba ka fetola ts'ebetso ho arola likarolo tse itseng tse khahlisang ka katleho, e leng se lebisang chaing e phahameng le bohloeki.

Mefuta ea Li-Centrifuge le Tšebeliso ea Tsona

Li-centrifuge li fumaneha ka mefuta e fapaneng, e 'ngoe le e 'ngoe e etselitsoe lits'ebetso tse itseng. Mefuta e sebelisoang haholo e kenyelletsa li-ultra-centrifuge, li-microcentrifuge, li-benchtop centrifuge, le li-preparative centrifuge.

- Li-centrifuge tse matla haholo: Mechini ena e matla e khona ho hlahisa lebelo le phahameng haholo, hangata e fihla ho li-revolutions tse fetang 100,000 ka motsotso (RPM). Li sebelisoa lipatlisisong tsa biochemical, ho latellana ha DNA, le tlhahisong ea meriana.

- Microcentrifuges: Li-centrifuge tsena tse nyane li na le bokhoni bo tlase empa li loketse liteko tse nyane lilaboratoring. Li sebelisoa bakeng sa ho lokisa sampole ea DNA le RNA, setso sa sele le tlhahlobo ea protheine.

- Benchtop centrifuges: E tsebahala ka ho tenyetseha ha eona le ho ba bonolo ho e sebelisa, benchtop centrifuges e fumana lits'ebetso lilaboratoring tsa bongaka, biochemistry le biotechnology. Li loketse ho lokisoa ha sampole ka kakaretso, karohano ea likarolo tsa mali le tlhoekiso ea protheine.

- Mechini e lokisang: Mechini ena e matla e etselitsoe ka ho khetheha mesebetsi ea indasteri e meholo. Hangata e fumanoa liindastering tsa lik'hemik'hale le tsa baeloji bakeng sa lits'ebetso tse kang tlhahiso ea ente, kotulo ea lisele le tlhoekiso e kholo ea protheine.

Litšebeliso tsa Indasteri le Saense tsa Centrifugation

Ho centrifugation ho bapala karolo ea bohlokoa liindastering tse ngata, ho kenyeletsoa meriana, lijo le lino, saense ea tikoloho le lik'hemik'hale tsa petrochemical.

Indastering ea meriana, ho sebelisa centrifugation ho sebelisoa nakong ea nts'etsopele le tlhahiso ea meriana, ho thusa ho arola metsoako e sebetsang ea meriana (API) ho litšila, lithōle tsa sele le lintho tse ling tse sa batleheng. E boetse e sebelisoa bakeng sa ho arola likarolo tsa mali libankeng tsa mali, e leng se nolofalletsang ho ntšoa ha plasma, lisele tse khubelu tsa mali le li-platelet bakeng sa mekhoa e fapaneng ea bongaka.

Ka hare ho indasteri ea lijo le lino, centrifugation e thusa ho hlakisa metsi, joalo ka lero la litholoana, veine le biri, ho tlosa marotholi le ho ntlafatsa ho hlaka ha sehlahisoa. E boetse e sebelisoa tlhahisong ea oli ea mohloaare, e arola oli ho metsi le lintho tse tiileng. Ho feta moo, e bapala karolo ea bohlokoa ts'ebetsong ea lebese, e nolofalletsang karohano ea tranelate ho lebese.

Lefapheng la mahlale a tikoloho, ho centrifugation ho bohlokoa haholo ho hlahlobeng disampole tsa mobu, kalafo ea metsi a litšila, le ho ithuta likokoana-hloko tse phelang metsing. E thusa ho arola likaroloana tse tiileng metsing, e thusa tlhahlobo e nepahetseng le ho beha leihlo litšila tsa tikoloho.

Indasteri ea petrochemical e sebelisa haholo centrifugation ho arola metsoako ea oli e tala, ho hloekisa litlolo, le ho ntlafatsa katleho ea ho hloekisa oli. Liteko tsa taolo ea boleng le mesebetsi ea lipatlisiso le nts'etsopele li rua molemo ho tsoa mekhoeng ea centrifugation.

Likotsi le Meeli ea Karohano ea Centrifugal

Leha ho centrifugation e le mokhoa o matla le o ke keng oa hlokoa, o na le likotsi le mefokolo e itseng e lokelang ho nahanoa. Ho centrifugation ka lebelo le phahameng ho ka hlahisa mocheso o moholo, o ka amang botšepehi ba sampole kapa oa fetola botsitso ba metsoako ea baeloji. Ho feta moo, ho seta ka tsela e fosahetseng lipeipi kapa li-rotor, ho hloleha ho leka-lekanya lisampole, kapa ho feta lebelo le phahameng le khothaletsoang ho ka fella ka ho hloleha ha lisebelisoa, ho beha likotsi tsa polokeho.

Moedi o mong o kenyeletsa monyetla oa ho silafatsoa ha ho sebetsoa lisampole tse ngata ka rotor e le 'ngoe kapa ho hloleha ho hloekisa centrifuge hantle lipakeng tsa ho matha. Tšilafalo ena e ka lebisa liphethong tse fosahetseng tsa liteko kapa bohloeki bo fokotsehileng ba lisampole.

Ho phaella moo, dintho tse itseng di kanna tsa se tsamaisane le centrifugation ka lebaka la thepa ya tsona kapa kutloelo-bohloko. Disampole tse utlwang mocheso, metswako e feto-fetohang, kapa thepa ya baeloji e senyehang habonolo di ka hloka mekgwa e meng ya karohano ho netefatsa botsitso ba tsona.

Qetellong, ho centrifugation ke ts'ebetso ea bohlokoa e sebelisoang liindastering tse fapaneng le mafapheng a saense. Ho utloisisa saense e ka morao ho karohano ea centrifuge, ho sebelisa mofuta o loketseng oa centrifuge, le ho nahana ka mefokolo e amanang le mokhoa ona ho bohlokoa bakeng sa karohano e atlehileng, tlhoekiso le tlhahlobo ea lintho tse fapaneng.