motheo oa karohano ho sebelisoa centrifuge ke ofe?

Selelekela:

Li-centrifuge ke lisebelisoa tsa bohlokoa tsa laboratori tse sebelisetsoang ho arola lintho ho latela phapang ea tsona ea bongata litharollong tsa mokelikeli. Ka bokhoni ba tsona ba ho potoloha lisampole ka potlako ka lebelo le phahameng, li-centrifuge li fetotse masimo a fapaneng, ho tloha tlhokomelong ea bophelo bo botle le meriana ho ea ho baeloji le k'hemistri. Sehlooho sena se ikemiselitse ho hlahloba melao-motheo le mekhoa e ka morao ho karohano ho sebelisoa theknoloji ea centrifuge, ho senola lefatše le khahlang le ka har'a mechini ena e matla.

I. Centrifuge: Sesebelisoa sa Motheo ho Saense

II. Molao-motheo oa Centrifugation

III. Mefuta ea Li-Centrifuge

IV. Litšebeliso tsa Centrifugation

V. Tsoelo-pele Theknolojing ea Centrifuge

I. Centrifuge: Sesebelisoa sa Motheo ho Saense

Di-centrifuge ke karolo ea bohlokoa ea lipatlisiso tsa mahlale le lits'ebetso tsa nts'etsopele. Li lumella bo-rasaense ho arola, ho hloekisa le ho sekaseka mefuta e mengata ea lisampole, ho kenyeletsoa le maro a baeloji, lisele, limolek'hule, esita le likaroloana tse nyane. Ka ho sebelisa melao-motheo ea ho kopanya, lisebelisoa tsena li arola likarolo tse itseng ka har'a motsoako o fapaneng, e leng se nolofalletsang bafuputsi ho ithuta le ho li utloisisa hamolemo.

II. Molao-motheo oa Centrifugation

Ho centrifugation ho itshetlehile ka mohopolo wa ho sedimentation, o hlahang ka lebaka la bongata bo fapaneng ba dikarolwana tse leketlileng moyeng o metsi. Ha sampole e kenngwa ka hara rotor ya centrifuge mme e potoloha ka potlako, matla a centrifugal a sebediswa motswakong. Matla ana a sutumelletsa dikarolwana tse teteaneng ho ya tlase ho tharollo, a etsa pellet, ha dikarolwana tse bobebe di ntse di le ka hara supernatant.

III. Mefuta ea Li-Centrifuge

1. Di-Centrifuge tse Fapaneng:

Di-centrifuge tse fapaneng di sebetsa ka lebelo le tlase ho feta mefuta e meng empa di loketse ho arola dikarolwana tse kgolo kapa dikarolo tsa sele ho di-suspension kapa di-lysate.

2. Li-Centrifuge tsa ho Lokisetsa:

Li-centrifuge tse lokisetsang li ka sebetsana le bongata bo boholo ba sampole ka lebelo le phahameng ho feta li-centrifuge tse fapaneng. Li lumella karohano e potlakileng le e sebetsang haholoanyane, e leng se etsang hore li lokele lits'ebetso tse kholo tsa tlhoekiso.

3. Li-Ultracentrifuge:

Li-ultracentrifuge ke mesebetsi e boima ea saense ea karohano. Mechini ena e sebetsa ka lebelo le mocheso o phahameng haholo, e khona ho arola esita le limolek'hule tse ntle ka ho fetisisa kapa likaroloana tse ka tlas'a sele. Hangata li-ultracentrifuge li sebelisoa lipatlisisong tsa biochemical, e leng se thusang ho arola li-organelle le li-complex tse kholo.

IV. Litšebeliso tsa Centrifugation

1. Litšebeliso tsa Bongaka le tsa Bongaka:

Li-centrifuge li sebelisoa haholo lilaboratoring tsa bongaka ho sekaseka mali, moroto le maro a mang a 'mele. Litekong tsa ho hlahloba, li arola lisele ho serum kapa plasma ho khetholla lintho tse sa tloaelehang kapa ho fumana likarolo tse fapaneng tsa mali.

2. Indasteri ea Meriana:

Ho centrifugation ho bapala karolo ea bohlokoa ho sibolloeng le nts'etsopele ea meriana. Ho thusa ho aroleng le ho hloekiseng metsoako e sebetsang ea meriana, ho lumella tlhahlobo le tlhahiso e eketsehileng. Ho feta moo, centrifuge e sebelisoa nakong ea tlhahiso ea liente, ho tlosa likaroloana tsa vaerase kapa ho hloekisa liprotheine tse kopantsoeng hape.

3. Tlhahlobo ea Tikoloho:

Saenseng ea tikoloho, ho centrifugation ho molemo bakeng sa ho sekaseka lisampole tsa mobu le metsi. Ho arola likaroloana tse emisitsoeng ho tsoa ho matrices ea tikoloho ho nolofalletsa bafuputsi ho ithuta mekhoa ea ho senyeha ha mobu, ho lekola maemo a tšilafalo, le ho sekaseka ho fumaneha ha limatlafatsi.

4. Baeloji ea Limolek'hule le Liphatsa tsa Lefutso:

Mekhoa ea ho centrifugation ke ea bohlokoa lipatlisisong tsa baeloji ea limolek'hule. Nakong ea ho ntšoa ha DNA, centrifuge e arola lisele ho tsoa mecheng ea tsona e li potolohileng, e leng se lumellang ho lysis le ho hloekisoa ha lintho tsa lefutso ka mor'a moo. Ho feta moo, ho aroloa ha likarolo tse ka tlas'a sele, tse kang mitochondria kapa ribosomes, ho thusa ho ithuta lits'ebetso tsa sele le tlhahiso ea protheine.

V. Tsoelo-pele Theknolojing ea Centrifuge

1. Li-Microcentrifuge:

Li-Microcentrifuge ke lisebelisoa tse nyane, tse lebelo le phahameng tse sebelisoang haholo-holo bakeng sa li-sampole tse nyane. Tsoelo-pele theknolojing ea microcentrifuge e lebisitse ho mechini e menyenyane, e khutsitseng le e bobebe haholoanyane, e leng se etsang hore e be sesebelisoa sa bohlokoa bakeng sa lipatlisiso sebakeng seo kapa tšimong.

2. Ho Fokotsa Phallo e Tsoelang Pele:

Di-centrifuge tsa setso di sebetsa ka mokgwa wa sehlopha, empa dintlafatso tsa moraorao di hlahisitse ho potoloha ha phallo e tswelang pele. Ditsamaiso tsena di dumella tlhahlobo le karohano ya nako ya sebele, ho ntlafatsa bokgoni le ho fokotsa dinako tsa tshebetso, haholoholo ditshebedisong tsa indasteri.

3. Boiketsetso bo Bohlale:

Ka ho kopanngoa ha litsamaiso tsa khomphutha, li-centrifuge tsa sejoale-joale li fana ka bokhoni bo bohlale ba ho iketsetsa. Ho tloha ho potlakiso e hlophisitsoeng le ho fokotseha ha motlakase ho isa ho taolo e nepahetseng ea mocheso, tsoelo-pele ena e netefatsa maemo a karohano le ho ikatisa ha ka nako e ts'oanang e fokotsa liphoso tsa mosebelisi.

4. Ho etsa hore e be nyane:

Tsoelo-pele mekhoeng ea ho etsa lintho tse nyenyane e nolofalitse nts'etsopele ea li-centrifuge tse nyane, tse tsejoang hape e le lisebelisoa tsa laboratori. Mekhoa ena e nkehang habonolo e lumella mekhoa ea karohano ho etsoa ka tekanyo e nyane, e bula menyetla e mecha ea ho fumana litlhahlobo tsa tlhokomelo le meriana e ikhethileng.

Qetello:

Li-centrifuge li fetotse lithuto tse ngata tsa mahlale ka ho nolofalletsa karohano e sebetsang le tlhahlobo ea lisampole tse fapaneng. Ka ho utloisisa melao-motheo e ka morao ho centrifuge le ho lula u tseba tsoelo-pele ea morao-rao theknolojing ea centrifuge, bafuputsi ba ka sibolla temohisiso le lits'ebetso tse ncha tse se nang palo masimong a fapaneng. Ha li-centrifuge li ntse li tsoela pele ho fetoha, tšusumetso ea tsona ho sibolloeng ha saense le boqapi e tla atleha ntle ho pelaelo.