Centrifugation e sebelisetsoang ho arola ke eng

Ho Centrifugation: Ho Arola Likarolo tsa Tsoelo-pele ea Saense

Selelekela

Ho centrifugation ke mokhoa o sebelisoang haholo lipatlisisong tsa saense le lits'ebetsong tsa indasteri o kenyeletsang ho arola likarolo tsa motsoako ka ho sebelisa matla a centrifugal. Ka ho sebelisa matla ana, bo-rasaense ba ka arola likaroloana ho latela boholo ba tsona, bongata le sebopeho, e leng se ba nolofalletsang ho ithuta le ho sekaseka karolo ka 'ngoe ka bonngoe. Sengoloa sena se tla hlahloba lits'ebetso tse fapaneng tsa ho centrifugation, se totobatsa kamoo e fetotseng masimo a kang baeloji, k'hemistri le bongaka kateng.

I. Molao-motheo o ka Morao ho Centrifugation

Ho utloisisa hore na ho centrifugation ho sebetsa joang, ho bohlokoa ho utloisisa molao-motheo oa motheo. Matla a centrifugal, a bonoang e le ho sutumelletsa kantle ho tloha bohareng, a etsoa ha motsoako o potoloha ka lebelo le phahameng ka har'a centrifuge. Matla ana a sebetsa holim'a likaroloana tse ka har'a motsoako, a etsa hore li tlohe bohareng ba potoloho. Ka lebaka leo, likaroloana tse nang le boholo le bongata bo fapaneng lia arohana 'me li bokellana maemong a fapaneng ka har'a sejana sa centrifugation.

II. Ho Arola Likarolo tsa Mali: Hematology

Tšebeliso e 'ngoe ea mantlha ea centrifugation ke lefapheng la hematology, moo likarolo tsa mali li aroloang bakeng sa merero ea ho hlahloba. Ha mali a kenngoa tlas'a matla a centrifugal, a arohana ka likarolo tse tharo tse kholo: plasma, leukocyte (lisele tse tšoeu tsa mali), le erythrocyte (lisele tse khubelu tsa mali). Karohano ena e lumella litsebi tsa bongaka ho lemoha lintho tse sa tloaelehang, ho ithuta mafu le ho beha leihlo maemo a bophelo ba bakuli.

III. Ho Hloekisa Limolek'hule tsa Bio: Biotechnology

Ho centrifugation ho bapala karolo ea bohlokoa theknolojing ea baeloji, moo ho sebelisetsoang ho hloekisa limolek'hule tse kang liprotheine le li-nucleic acid. Ka ho sebelisa mefuta e fapaneng ea mekhoa ea ho centrifugation, ho kenyeletsoa le ho centrifugation ka ho fetisisa, bo-rasaense ba ka arola limolek'hule ho tsoa metswakong e rarahaneng, joalo ka li-lysate tsa sele. Ts'ebetso ena ea tlhoekiso e bohlokoa bakeng sa tlhahlobo e latelang, ho sibolloa ha meriana le tlhahiso ea liprotheine tse kopantsoeng hape.

IV. Ho Arola Likarolo Patlisisong ea Tikoloho

Ho kenya centrifugation ho bohlokoa haholo lipatlisisong tsa tikoloho, haholo-holo ho ithuteng disampole tsa metsi le mobu. Ka ho beha disampole tsena tlas'a matla a centrifugation, likaroloana tse kang lintho tse tiileng tse emisitsoeng, lintho tse phelang le litšepe tse boima li ka aroloa ka katleho. Karohano ena e fana ka temohisiso ea bohlokoa mabapi le sebopeho le boleng ba tikoloho, e thusa bo-rasaense ho lekola maemo a tšilafalo le ho rala maano a ho lokisa bothata.

V. Ho Fokotsa Botenya ba Gradient: Ho Ithuta Dibopeho tse ka Tlase ho Sele

Ho centrifugation ha gradient ka bongata ke mokhoa o matla o dumellang karohano le karohano ya dibopeho tse ka tlase ho sele. Ka ho theha gradient e eketsang bongata ho tloha hodimo ho ya tlase ka hara tube, dikarolo tse fapaneng tsa sele, tse kang di-organelle kapa divaerase, di ka arolwa ho latela bongata ba tsona bo phaphametseng. Mokgwa ona o kentse letsoho haholo ho utlwisiseng tshebetso ya sele, thuto ya mafu a tshwaetsanwang, le ntshetsopele ya dikalafo tse ntjha.

VI. Litšebeliso tsa Liindasteri: Mekhoa ea ho Hloekisa le ho Arohanya

Ho centrifugation ho sebelisoa haholo makaleng a fapaneng a indasteri, ho kenyeletsoa meriana, ts'ebetso ea lijo le kalafo ea metsi a litšila. Indastering ea meriana, ho centrifugation ho sebelisoa bakeng sa lits'ebetso tse holimo le tse tlase. Ho bohlokoa bakeng sa ho arola lisele nakong ea ho belisoa, ho arola sehlahisoa ho lithōle tsa sele, le ho hloekisa lithethefatsi. Ts'ebetsong ea lijo, ho centrifugation ho thusa ho arola lintho tse tiileng, metsi le oli, ho netefatsa tlhahiso ea lihlahisoa tsa boleng bo holimo. Litsi tsa kalafo ea metsi a litšila li sebelisa centrifugation ho arola seretse ho metsi, ho nolofatsa phekolo le ho lahla metsi ka katleho.

Qetello

Ho sebelisa centrifugation ho fetotse lipatlisiso tsa saense le lits'ebetso tsa indasteri ka ho nolofalletsa karohano ea likarolo ka har'a motsoako. Ho tenyetseha ha mokhoa ona le mefuta e mengata ea lits'ebetso li bakile tsela ea tsoelo-pele masimong a fapaneng, ho tloha ho baeloji le k'hemistri ho ea ho bongaka le tlhahiso ea indasteri. Ha theknoloji e ntse e tsoela pele, mekhoa ea ho sebelisa centrifugation e tla fetoha ntle ho pelaelo, e fane ka ho nepahala le bokhoni bo boholo karohanong le tlhahlobong ea metsoako e fapaneng e rarahaneng.