chii chinonzi centrifugation chinoshandiswa kuparadzanisa

Kuparadzanisa Zvinhu Zvinoita Kuti Sainzi Ifambire Mberi: Kuparadzanisa Zvinhu Zvinoita Kuti Sainzi Ifambire Mberi

Nhanganyaya

Kubatanidza zvinhu (centrifugation) inzira inoshandiswa zvakanyanya mukutsvagisa kwesainzi uye maitiro emaindasitiri inosanganisira kupatsanura zvikamu zvemusanganiswa uchishandisa simba repakati nepakati. Nekushandisa simba iri, masayendisiti anogona kupatsanura zvidimbu zvichienderana nehukuru hwazvo, kuwanda kwazvo, uye chimiro, zvichivagonesa kudzidza nekuongorora chikamu chimwe nechimwe chimwe nechimwe. Chinyorwa chino chichaongorora mashandisirwo akasiyana-siyana ekubatanidza zvinhu (centrifugation), zvichiratidza kuti zvakachinja sei minda yakaita sebiology, chemistry, uye mishonga.

I. Nheyo Iri Kuseri Kwekudzora Kutenderera

Kuti unzwisise mashandiro anoita centrifugation, zvakakosha kunzwisisa musimboti uripo. Simba reCentrifugation, iro rinowanikwa sekusundidzira kwekunze kubva pakati, rinogadzirwa kana musanganiswa ukatenderedzwa nekumhanya kwakanyanya mu centrifuge. Simba iri rinoshanda pazvikamu zviri mukati memusanganiswa, zvichiita kuti zvibve pakati pekutenderera. Nekuda kweizvozvo, zvikamu zvine saizi dzakasiyana uye densities zvinoparadzana uye zvinoungana munzvimbo dzakasiyana mukati memudziyo we centrifugation.

II. Kuparadzanisa Zvikamu zveRopa: Hematology

Imwe nzira huru yekushandiswa kwe centrifugation ndeye hematology, uko zvikamu zveropa zvinoparadzaniswa kuti zviongororwe. Kana ropa rikaitwa nesimba re centrifugal, rinopatsanurwa kuita zvikamu zvitatu zvikuru: plasma, leukocytes (masero machena eropa), uye erythrocytes (masero matsvuku eropa). Kupatsanurwa uku kunobvumira vanachiremba kuona zvisina kunaka, kudzidza zvirwere, uye kutarisa mamiriro ehutano hwevarwere.

III. Kuchenesa Biomolecules: Biotechnology

Kugadzira centrifugation kunoita basa guru mu biotechnology, uko kunoshandiswa pakuchenesa biomolecules dzakadai semapuroteni nema nucleic acids. Nekushandisa nzira dzakasiyana dzekugadzira centrifugation, kusanganisira ultracentrifugation, masayendisiti anogona kuparadzanisa biomolecules kubva mumisanganiswa yakaoma, yakadai se cell lysates. Maitiro aya ekuchenesa akakosha pakuongorora kunotevera, kuwanikwa kwemishonga, uye kugadzirwa kwemapuroteni akabatana.

IV. Kuparadzanisa Zvikamu muKutsvagurudza Kwezvakatipoteredza

Kubatanidza mvura nevhu kunobatsira zvikuru mukutsvagisa kwezvakatipoteredza, kunyanya pakudzidza mvura nevhu. Nekuisa mienzaniso iyi pasi pesimba repakati nepakati, zvidimbu zvakaita sezvinhu zvakasungirirwa, zvinhu zvipenyu, uye simbi dzinorema zvinogona kupatsanurwa zvinobudirira. Kupatsanurwa uku kunopa ruzivo rwakakosha pamusoro pekuumbwa uye kunaka kwezvakatipoteredza, zvichibatsira masayendisiti kuongorora huwandu hwekusvibiswa uye kugadzira nzira dzekugadzirisa.

V. Density Gradient Centrifugation: Kudzidza Zvivako zveSubcellular

Kudzora huwandu hwemasero mumasero inzira ine simba inobvumira kupatsanurwa nekuparadzaniswa kwezvikamu zvemasero ari pasi pemasero. Nekugadzira kupatsanurwa kunowedzera kuwanda kubva kumusoro kusvika pasi mukati mechubhu, zvikamu zvakasiyana zvesero, zvakaita semaorganelles kana mavhairasi, zvinogona kupatsanurwa zvichienderana nekuwanda kwazvo. Nzira iyi yakabatsira zvikuru mukunzwisisa mashandiro emasero, kudzidza zvirwere zvinotapukira, uye kukura kwemishonga mitsva.

VI. Mashandisirwo Emaindasitiri: Maitiro Ekuchenesa Uye Kuparadzanisa

Kuchenesa mvura kunoshandiswa zvakanyanya mumaindasitiri akasiyana-siyana, kusanganisira mishonga, kugadzira chikafu, uye kurapa mvura yakasviba. Muindasitiri yemishonga, kufukidza mvura kunoshandiswa pakuita zvinhu zviri kumusoro nepasi. Zvakakosha pakuparadzanisa masero panguva yekuvirisa, kuparadzanisa chigadzirwa kubva mumarara emasero, uye kuchenesa mishonga. Mukugadzira chikafu, kuparadzanisa mvura kunobatsira kuparadzanisa zvinhu zvakasimba, zvinwiwa, nemafuta, zvichiita kuti zvigadzirwa zvemhando yepamusoro zvigadzirwe. Mafekitori ekuchenesa mvura yakasviba anoshandisa kuparadzanisa tsvina kubva mumvura, zvichiita kuti kurapwa nekurasa tsvina zvive nyore.

Mhedziso

Kugadzira simbi ye "centrifugation" kwakachinja kutsvagurudza kwesainzi uye maitiro emaindasitiri nekugonesa kupatsanurwa kwezvikamu mumusanganiswa. Kushanda nesimba kwehunyanzvi uhwu uye mashandisirwo akasiyana-siyana kwakavhura nzira yekufambira mberi muzvikamu zvakasiyana-siyana, kubva kubiology nekemesitiri kusvika kumishonga nekugadzira maindasitiri. Sezvo tekinoroji ichiramba ichifambira mberi, matekiniki ekugadzira simbi ye "centrifugation" achachinja pasina mubvunzo, zvichipa kunyatsojeka uye kushanda zvakanaka mukupatsanura nekuongorora misanganiswa yakasiyana-siyana yakaoma.