karohano ea centrifugation ke eng

Karohano ea Centrifugation ke eng?

Karohano ea centrifugation ke mokhoa o sebelisoang haholo o sebelisang molao-motheo oa matla a centrifugation ho arola likarolo tse fapaneng tsa motsoako. Mokhoa ona o sebelisoa masimong a fapaneng, ho kenyeletsoa lilaboratori tsa lipatlisiso, lipetlele, liindasteri tsa tlhahiso, esita le lifemeng tsa kalafo ea metsi a litšila. Ka ho beha sampole ho potoloha ka lebelo le phahameng, centrifugation e arola likarolo ho latela boholo ba tsona, bongata le sebopeho. Ke mokhoa o matla le o sebetsang hantle o fanang ka melemo e meholo ho feta mekhoa e meng e tloaelehileng ea karohano.

Ho Utloisisa Melao-motheo ea Centrifugation

Ho utloisisa ka botlalo karohano ea centrifuge, ho bohlokoa ho utloisisa melao-motheo ea motheo ea mokhoa ona. Ha motsoako o kenngoa ka har'a centrifuge, o potoloha ka potlako ho potoloha axis e bohareng, o hlahisa matla a matla a centrifuge. Matla ana a etsa hore likaroloana tse boima kapa lintho li tsamaee libakeng tse ka ntle tsa motsoako, ha likarolo tse bobebe li lula li le haufi le bohareng. Karohano ena ea sebaka e lumella pokello ea likarolo ka bomong, e thusang ho hloekisa, ho sekaseka le ho hlalosa mekhoa.

Bohlokoa ba ho Centrifugation Lipatlisisong tsa Baeloji

Karohano ea Lisele tse ka Tlase: Tšebeliso e 'ngoe ea bohlokoa ea karohano ea centrifugation lipatlisisong tsa baeloji ke karohano ea lisele tse ka tlase ho sele. Ka ho taboha lisele ka bonolo le ho li beha tlas'a matla a centrifugal, bafuputsi ba ka arola likarolo tse itseng tsa sele tse kang li-organelle, liprotheine le li-nucleic acid. Mokhoa ona o bohlokoa bakeng sa ho ithuta meaho ea sele, ho etsa liteko tsa enzymatic, le ho batlisisa likamano tsa protheine-protein kapa protheine-DNA.

Ho Pelletsoa ha Limolek'hule: Ho pelletsoa ha limolek'hule ho boetse ho bohlokoa ho aroleng le ho bokelleng limolek'hule tsa limolek'hule, ho kenyeletsoa le DNA, RNA le liprotheine. Ka ho sebelisa mekhoa ea ho pelletsoa ha limolek'hule tsa ultracentrifugation, limolek'hule tsena tsa limolek'hule li ka pelletsoa ho tsoa motswakong, ho nolofatsa tlhoekiso ea tsona le tlhahlobo e latelang. Sena se nolofalletsa bo-rasaense ho ithuta le ho utloisisa sebopeho, mosebetsi le boitšoaro ba metsoako ena ea bohlokoa ea baeloji.

Likopo tsa Bongaka

Teko ea Tlhahlobo: Ho thiba lisele tsa mali ho phetha karolo ea bohlokoa tikolohong ea laboratori ea bongaka, ho thusa litekong tsa tlhahlobo. Ebang ke ho sekaseka lisampole tsa mali bakeng sa matšoao a mafu kapa ho khetholla likokoana-hloko marong a 'mele, ho thiba lisele tsa mali ho thusa ho arola bahlahlobi ba shebiloeng ho lintho tse sitisang. Karohano e hlakileng e fihlellehang ka ho thiba lisele tsa mali e ntlafatsa ho nepahala le botšepehi ba liphetho tsa tlhahlobo, e leng se nolofalletsang litsebi tsa tlhokomelo ea bophelo ho etsa liqeto tse nang le tsebo tsa bongaka.

Karohano ea Serum le Plasma: Tlhahlobong ea bongaka, serum le plasma ke likarolo tsa bohlokoa bakeng sa liteko tse fapaneng kaha li na le tlhaiso-leseling ea bohlokoa ea biochemical. Centrifugation e nolofalletsa karohano e sebetsang ea serum kapa plasma maling 'ohle, e lumellang liteko tse felletseng tsa tlhahlobo. Karohano ena e bohlokoa bakeng sa liteko tse kang tlhahlobo ea lipid, liteko tsa ts'ebetso ea sebete, le tlhahlobo ea lihormone, har'a tse ling.

Likopo tsa Liindasteri

Karohano ea Oli le Metsi: Ho sebelisa centrifugation hangata ho sebelisoa liindastering tse sebetsanang le metsoako ea oli le metsi, joalo ka libaka tsa ho hloekisa peterole le lifeme tsa kalafo ea metsi a litšila. Ka ho sebelisa matla a centrifugal, likarolo tsa oli le metsi li ka aroloa ka katleho, e leng se nolofalletsang ho hlaphoheloa ha oli ea bohlokoa le ho fokotsa likotsi tsa tšilafalo ea tikoloho. Ho sebelisa centrifugation ho fana ka tharollo e theko e tlaase le e bolokang matla liindastering tsena.

Karohano ea Metsi a Tiileng: Mekhoa e mengata ea tlhahiso e hlahisa metsoako e nang le likaroloana tse tiileng tse leketlileng mokerong oa metsi. Karohano ea centrifugation e fana ka mokhoa o atlehang oa ho arola likarolo tsena, e leng se nolofalletsang ho fumana lintho tse tiileng tsa bohlokoa kapa ho hloekisa lintho tse metsi. Litšebeliso li ka fumanoa liindastering tse fapaneng, ho kenyeletsoa meriana, lijo le lino, le tlhahiso ea lik'hemik'hale.

Qetello

Karohano ea centrifugation ke mokhoa o matla o fumanang lits'ebetso mafapheng a fapaneng, ho tloha lipatlisisong tsa baeloji ho ea ho tlhahlobo ea bongaka le lits'ebetso tsa indasteri. Ka ho sebelisa melao-motheo ea matla a centrifugation, mokhoa ona o nolofalletsa karohano ea metsoako ka likarolo tse fapaneng ho latela thepa ea tsona ea 'mele. Karohano ea li-subcellular, karohano ea limolek'hule, liteko tsa tlhahlobo, karohano ea metsi a oli, le karohano ea metsi a tiileng ke mehlala e 'maloa feela ea lits'ebetso tse ngata tsa karohano ea centrifugation. Ka ho arola likarolo ka katleho, mokhoa ona o nolofalletsa tlhoekiso e sebetsang, tlhahlobo le tlhaloso, qetellong e kenya letsoho tsoelo-peleng ea lipatlisiso, tlhahlobo le lits'ebetso tsa indasteri.