chii chinonzi centrifugation separation

Chii chinonzi Centrifugation Separation?

Kuparadzanisa centrifugation inzira inoshandiswa zvakanyanya inoshandisa simba re centrifugal kuparadzanisa zvikamu zvakasiyana zvemusanganiswa. Nzira iyi inoshandiswa munzvimbo dzakasiyana-siyana, kusanganisira marabhoritari ekutsvagisa, zvipatara, maindasitiri ekugadzira, uye kunyangwe mumafekitori ekuchenesa mvura yakasviba. Nekuisa sampuro pakutenderera nekukurumidza, centrifugation inoparadzanisa zvikamu zvichienderana nehukuru hwazvo, huwandu hwazvo, uye chimiro. Iyi inzira ine simba uye inoshanda inopa mabhenefiti makuru pane dzimwe nzira dzechinyakare dzekuparadzanisa.

Kunzwisisa Nheyo dzeCentrifugation

Kuti unzwisise zvizere kupatsanurwa kwe centrifugation, zvakakosha kunzwisisa misimboti iri pasi peiyi nzira. Kana musanganiswa ukaiswa mu centrifuge, unotenderera nekukurumidza kutenderedza central axis, zvichigadzira simba rakasimba re centrifugation. Simba iri rinoita kuti zvidimbu zvinorema kana zvinhu zvifambe kuenda kunzvimbo dzekunze dzemusanganiswa, nepo zvikamu zvakareruka zvichiramba zviri pedyo nepakati. Kupatsanurwa uku kwenzvimbo kunobvumira kuunganidzwa kwezvikamu zvakasiyana, zvichibatsira mukuchenesa, kuongorora, uye maitiro ekutarisa.

Kukosha kweCentrifugation muBiological Research

Kupatsanurwa kwemasero ari pasi pemasero: Imwe nzira yakakosha yekupatsanurwa kwemasero ari pasi pemasero muongororo yezvebhayoloji ndeyekuparurwa kwemasero ari pasi pemasero. Nekupwanya masero zvinyoro nyoro uye kuaisa pasi pesimba remasero ari pasi pemasero, vaongorori vanogona kusiyanisa zvikamu zvemasero zvakaita se organelles, mapuroteni, uye nucleic acids. Iyi nzira yakakosha pakudzidza maumbirwo emasero, kuita bvunzo dze enzyme, uye kuferefeta kudyidzana kwemapuroteni-mapuroteni kana mapuroteni-DNA.

Kubvisa maBiomolecules: Kubvisa ma centrifugation kunokoshawo pakuparadzanisa nekuunganidza ma biomolecules, kusanganisira DNA, RNA, uye mapuroteni. Uchishandisa nzira dzekubvisa ma ultracentrifugation, ma macromolecules aya anogona kubviswa kubva mumusanganiswa, zvichiita kuti acheneswe uye aongororwe zvinotevera. Izvi zvinoita kuti masayendisiti akwanise kudzidza nekunzwisisa chimiro, mashandiro, uye maitiro emakemikari aya akakosha ebhayoloji.

Maitiro Ekurapa

Kuongororwa Kwechirwere: Kuongororwa kwemasero eropa (centrifugation) kunoita basa rakakosha murabhoritari yekiriniki, zvichibatsira mukuongorora chirwere. Kungave kuongorora ropa rekuongorora zviratidzo zvechirwere kana kuona utachiona huri mumvura yemuviri, kuongororwa kwemasero eropa (centrifugation) kunobatsira kuparadzanisa vanoongorora chirwere kubva kune zvinhu zvinokanganisa. Kupatsanurwa kwakajeka kunowanikwa kuburikidza nekuongorora kwemasero eropa (centrifugation) kunowedzera kururama uye kuvimbika kwemhedzisiro yekuongorora chirwere, zvichiita kuti vashandi vezvehutano vakwanise kuita sarudzo dzekurapa dzine ruzivo.

Kupatsanurwa kweSerum nePlasma: Mukuongorora kwekurapa, serum neplasma zvinhu zvakakosha pakuongorora kwakasiyana-siyana sezvo zvine ruzivo rwakakosha rwe biochemical. Kupatsanurwa kwemasero kunoita kuti serum kana plasma iparadzaniswe zvakanaka kubva muropa rose, zvichibvumira kuongororwa kwakazara kwekuongorora. Kupatsanurwa uku kwakakosha pakuongorora kwakadai se lipid profiling, bvunzo dzekushanda kwechiropa, uye kuongororwa kwemahormone, pakati pezvimwe.

Zvikumbiro zveMaindasitiri

Kupatsanurwa kweOiri neMvura: Kupatsanurwa kwemhepo kunowanzo shandiswa mumaindasitiri anobata nemusanganiswa wemvura yemafuta, senge nzvimbo dzinochenesa peturu nemiti yekuchenesa mvura yakasviba. Nekushandisa simba remagetsi, zvikamu zvemafuta nemvura zvinogona kupatsanurwa zvinobudirira, zvichigonesa kudzoreredzwa kwemafuta anokosha uye kuderedza njodzi dzekusvibiswa kwezvakatipoteredza. Kupatsanurwa kwemhepo kunopa mhinduro inoshanda uye isingadhuri muindasitiri idzi.

Kupatsanurwa Kwemvura Yakasimba: Maitiro mazhinji ekugadzira anogadzira musanganiswa une zvidimbu zvakasimba zvakasungirirwa mune imwe nzira yemvura. Kupatsanurwa kwe centrifugation kunopa nzira inoshanda yekuparadzanisa zvikamu izvi, zvichiita kuti zvinhu zvakakosha zvidzoke kana kucheneswa kwezvinwiwa. Mashandisirwo anowanikwa mumaindasitiri akasiyana-siyana, anosanganisira mishonga, chikafu nezvinwiwa, uye kugadzira makemikari.

Mhedziso

Kupatsanurana kwemasero (centrifugation separation) inzira ine simba inoshandiswa muzvikamu zvakasiyana-siyana, kubva pakutsvagurudza kwezvipenyu kusvika pakuongorora zvirwere uye maitiro eindasitiri. Nekushandisa misimboti yesimba remagetsi (centrifugal force), nzira iyi inobatsira kupatsanurana kwemisanganiswa kuita zvikamu zvakasiyana zvichienderana nehunhu hwazvo hwepanyama. Kupatsanurana kwemasero, kupatsanurana kwemasero, kuyedzwa kwekuongorora, kupatsanurana kwemvura nemafuta, uye kupatsanurana kwemvura yakasimba inongova mienzaniso mishoma yekushandiswa kwakawanda kwekupatsanurana kwemasero. Nekupatsanurana zvinobudirira kwezvikamu, nzira iyi inobvumira kuchenesa, kuongorora, uye kutsanangura zvinhu zvinobudirira, zvichizobatsira mukufambira mberi mukutsvagurudza, kuongorora, uye maitiro eindasitiri.