mokhoa oa ho centrifuge mochining o nang le litlhophiso tse tlase ho feta tse hlokahalang

Ho Centrifugation: Mokhoa oa ho Ntlafatsa Ts'ebetso ea Mochini ka Litlhophiso tse Tlase

Ho Utloisisa ho Centrifugation le Bohlokoa ba Eona Liindastering tse Fapaneng

Mabaka a Susumetsang Tshebetso ya Centrifugation

Mathata a ho Sebetsa ha Li-Centrifuge ka Litlhophiso tse Tlase

Malebela a ho Centrifugation e Sebetsang ka Lebelo le Tlase

Ho netefatsa ho nepahala le polokeho ho Centrifugation ea lebelo le tlase

Selelekela:

Ho centrifugation ke ts'ebetso ea bohlokoa e sebelisoang haholo lilaboratoring, litsing tsa lipatlisiso, le libakeng tsa indasteri bakeng sa ho arola metsoako ho latela boholo ba likaroloana, bongata le ho teteana. Leha ho le joalo, ho na le linako tseo ho sebelisa mochini o nang le litlhophiso tse tlase ho hlokahalang ka lebaka la lithibelo tse itseng. Sehloohong sena, re tla hlahloba mokhoa oa ho centrifugation ka katleho re sebelisa litlhophiso tse tlase tsa mochini ha re ntse re fihlela liphetho tse tšepahalang, re boloka bokhoni, le ho netefatsa polokeho.

Ho Utloisisa ho Centrifugation le Bohlokoa ba Eona Liindastering tse Fapaneng:

Ho Centrifugation, mokhoa o qapiloeng ke rasaense oa Lefora Antoine Jérôme Balard lekholong la bo19 la lilemo, o sebelisa matla a centrifugal ho arola likaroloana tse leketlileng mokerong oa metsi. Ts'ebetso ena e na le bohlokoa bo boholo mafapheng a fapaneng a kang meriana, theknoloji ea baeloji, tlhahlobo ea bongaka le tlhahlobo ea tikoloho, moo e thusang ho arola le ho hloekisa lintho tse reriloeng.

Mabaka a Susumetsang Tshebetso ya Centrifugation:

Ho ntlafatsa ho centrifugation, ho utloisisa lintlha tse susumetsang ts'ebetso ea eona ho bohlokoa. Lintlha tsena li kenyelletsa boholo ba rotor le mofuta, bophahamo ba sampole le litšobotsi, mocheso, le, habohlokoa, lebelo la centrifuge. Leha lebelo le phahameng ka kakaretso le khethoa bakeng sa karohano e sebetsang hantle, ho na le maemo a hlokang ho sebelisoa litlhophiso tse tlase.

Mathata a ho Sebetsa ha Li-Centrifuge ka Litlhophiso tse Tlase:

Ho sebelisa centrifuge ka lebelo le tlase ho feta le khothaletsoang ho ka hlahisa liphephetso tse 'maloa. Ea pele, ho fihlella karohano e lekaneng ho nka nako e ngata, ho eketsa nako eohle ea ts'ebetso. Ho feta moo, ho na le kotsi ea karohano e sa fellang kapa phokotso ea ho hlaphoheloa ha likarolo tse lakatsehang, e leng se fokotsang ho nepahala ha teko.

Malebela a ho Centrifugation e Sebetsang ka Botlalo ka Lebelo le Tlase:

1. Kgetha Rotor le Boholo bo Loketseng ba Tube: Ho kgetha rotor le boholo ba tube bo loketseng tshebetso e potlakileng ho bohlokwa. Kgetho e ntle ya rotor e netefatsa tsamaiso e nepahetseng ya sampole mme e thusa karohano e sebetsang ho sa tsotelehe matla a tlase a centrifugal.

2. Fetola Nako le Mocheso: Ha ho sebelisoa centrifugation ka lebelo le tlase, ho fetola nako le mocheso ho ka buseletsa matla a khoheli a fokotsehileng. Ho fokotsa lebelo la ts'ebetso le ho eketsa mocheso hanyane ho ka ntlafatsa katleho ea karohano ha ho ntse ho bolokoa botšepehi ba sampole.

3. Sebelisa Lisebelisoa tsa ho Ntlafatsa Matlafatso ea Centrifugation: Ho latela mofuta oa sampole, ho kenyelletsa lintho tse ling tse eketsehileng kapa li-reagent tse itseng ho ka ntlafatsa ts'ebetso ea ho ntlafatsa matlafatso esita le ka lebelo le tlase. Tsena li ka kenyelletsa li-gradients tsa density, tse kang sucrose kapa iodixanol, tse thusang ho arola likaroloana kapa li-organelle tse itseng.

4. Ntlafatsa Tokisetso ea Sampole: Tokisetso e phethahetseng ea sampole e susumetsa haholo katleho ea ho centrifugation, haholo-holo ha ho sebetsanoa le maemo a tlase. Ho ngola le ho sebetsana le lisampole ka nepo, ho tlosa litšila, le ho sebelisa li-buffer kapa mecha ea phatlalatso e loketseng ho tla netefatsa liphetho tse tšepahalang le tse ka ikatisang hape.

5. Etsa Mabelo a Mangata kapa ho Ohla Pele ho Sepeiso: Haeba ho khoneha, ho etsa mabelo a mangata kapa ho etsa potoloho e khutšoanyane pele ho sepeiso ka lebelo le phahameng ho ka thusa ho hlola meedi e amanang le ho centrifugation ka lebelo le tlase. Mohato ona wa pele o ka tsepamisa sampole esale pele, wa thusa karohano e latelang nakong ya tshebetso ya lebelo le tlase.

Ho netefatsa ho nepahala le polokeho ha ho sebelisoa Centrifugation e lebelo le tlase:

Ha o ntse o sebedisa centrifuge maemong a tlase, ho bohlokwa ho boloka ho nepahala le polokeho. Mona ke dintlha tsa bohlokwa:

1. Leka-lekanya Lirotara tsa Swing-Bucket: Ho leka-lekanya rotara ea centrifuge ka ho aba liphaephe tsa sampole ka ho lekana libakeng tse fapaneng ho thusa ho fokotsa ho thothomela le ho thibela tšenyo ea lisebelisoa nakong ea ts'ebetso ea lebelo le tlase.

2. Lekola ho senngoa ha mashala le ho aroloa ha mobu: Lekola sampole khafetsa nakong ea ho senngoa ha mobu ho lekola tsoelo-pele ea ho senngoa ha mobu le karohano. Mohato ona o thusa ho fumana hore na liphetoho lia hlokahala ho fihlela liphetho tse ntle ka ho fetisisa.

3. Latela Mekhoa e Metle ea Laboratori (GLP): Ho latela litataiso tsa GLP, tse kang ho apara lisebelisoa tse loketseng tsa tšireletso ea botho (PPE), ho sebelisa litiiso tsa lipeipi tse nepahetseng, le ho ngola lisampole ka nepo, ho netefatsa polokeho le ho boloka botšepehi ba teko.

4. Sebetsa 'moho le Litsebi: Haeba u sebetsa ka lisampole tse sa tloaelehang kapa u tobane le liphephetso tse amanang le ho centrifugation ka lebelo le tlase, litsebi tsa boeletsi lefapheng lena, joalo ka bahlahisi ba centrifuge kapa litsebi tse nang le boiphihlelo, li ka fana ka temohisiso le tataiso ea bohlokoa.

Qetello:

Ho centrifugation maemong a tlase a mochini ho hloka ntlafatso e nahannweng ho hlola diphephetso le ho fihlella diphetho tse tshepahalang. Ka ho kgetha di-rotor tse loketseng ka hloko, ho fetola nako le mocheso, ho sebedisa di-enhancer tsa centrifugation, ho ntlafatsa boitokisetso ba sampole, le ho latela diprothokholo tsa polokeho, bafuputsi ba ka kgona ho tsamaya ka katleho ho centrifugation ka lebelo le tlase ha ba ntse ba boloka ho nepahala, bokgoni le polokeho.