maitiro ekuisa centrifuge mumuchina une zvigadziriso zvishoma pane zvinodiwa

Kudzora Kushanda Kwemuchina: Maitiro Ekugadzirisa Kushanda Kwemuchina Nezvirongwa Zvakaderera

Kunzwisisa Centrifugation uye Kukosha Kwayo Mumaindasitiri Akasiyana-siyana

Zvinhu Zvinokanganisa Kushanda kweCentrifugation

Matambudziko ekushandisa maCentrifuge neLower Settings

Mazano ekushandisa Centrifugation zvakanaka pakukurumidza kwakaderera

Kuve nechokwadi chekururama uye kuchengetedzeka muLow-Speed ​​​​Centrifugation

Nhanganyaya:

Kupinza zvinhu mu "centrifugation" inzira inokosha inoshandiswa zvakanyanya mumarabhoritari, nzvimbo dzekutsvagisa, uye munzvimbo dzemaindasitiri pakupatsanura musanganiswa zvichienderana nehukuru hwezvidimbu, huwandu, uye kusimba. Zvisinei, pane dzimwe nguva apo kushandisa muchina une zvigadziriso zvishoma kunodiwa nekuda kwezvirambidzo zvakati. Muchinyorwa chino, tichaongorora maitiro ekuisa zvinhu mu "centrifuge" zvinobudirira tichishandisa zvigadziriso zvemuchina zvakati wandei uku tichiri kuwana mhedzisiro yakavimbika, kuchengetedza kushanda zvakanaka, uye kuona kuchengetedzeka.

Kunzwisisa Kushandiswa Kwemagetsi Nekukosha Kwawo Mumaindasitiri Akasiyana-siyana:

Kugadzira simbi, nzira yakagadzirwa nesainzi wekuFrance Antoine Jérôme Balard muzana remakore rechi19, inoshandisa simba resimbi kuparadzanisa zvidimbu zvakasungirirwa mumvura. Maitiro aya akakosha zvikuru muzvikamu zvakasiyana-siyana zvakaita semishonga, biotechnology, kuongororwa kwekiriniki, uye kuongorora zvakatipoteredza, uko kunobatsira mukubvisa nekuchenesa zvinhu zvinotsvagwa.

Zvinhu Zvinokanganisa Kushanda kweCentrifugation:

Kuti centrifugation ishande zvakanaka, kunzwisisa zvinhu zvinopesvedzera mashandiro ayo kwakakosha. Zvinhu izvi zvinosanganisira saizi yerotor nerudzi rwayo, huwandu hwemuenzaniso uye hunhu hwayo, tembiricha, uye, zvakakosha, kumhanya kwecentrifuge. Kunyange hazvo kumhanya kwakakwirira kuchiwanzo shandiswa pakuparadzanisa zvinobudirira, kune mamiriro ezvinhu anoda kuti pave nezvirongwa zvakaderera.

Matambudziko ekushandisa maCentrifuge ane maLower Settings:

Kushandisa centrifuge nekumhanya kwakaderera pane kwakakurudzirwa kunogona kukonzera matambudziko akati wandei. Chekutanga, kuwana kupatsanurwa kwakakwana kunotora nguva yakawanda, zvichiwedzera nguva yekushanda. Pamusoro pezvo, pane njodzi yekupatsanurwa kusina kukwana kana kudzikira kwekudzoserwa kwezvinhu zvinodiwa, zvichideredza kururama kwekuyedza.

Mazano ekushandisa centrifugation zvakanaka nekumhanya kwakaderera:

1. Sarudza Rotor neChubhu Yakakodzera Saizi: Kusarudza rotor nechubhu saizi yakakodzera mashandiro akaderera kwakakosha. Kusarudza rotor yakakwana kunoita kuti sampuli ibatiswe zvakanaka uye kunoita kuti kupatsanurwa kubudirire pasinei nesimba rakaderera re centrifugal.

2. Gadzirisa Nguva neTembiricha: Mukushandiswa kwemhepo ine simba shoma, kugadzirisa nguva netembiricha kunogona kutsiva simba regiravhiti rakaderera. Kuderedza kumhanya kwemaitiro uye kuwedzera tembiricha zvishoma kunogona kuwedzera kushanda zvakanaka kwekuparadzanisa uku uchichengetedza kuvimbika kwemuenzaniso.

3. Shandisa Zvinowedzera Kushanda Kwema "Centrifugation Enhancers": Zvichienderana nerudzi rwemuenzaniso, kuisa zvimwe zvinowedzerwa kana ma "reagents" zvinogona kuvandudza mashandiro e "centrifugation" kunyangwe paine kumhanya kwakaderera. Izvi zvinogona kusanganisira density gradients, dzakadai se sucrose kana iodixanol, iyo inobatsira mukubvisa zvidimbu kana ma "organelles" chaiwo.

4. Gadzirisa Kugadzirira Sampuro: Kugadzirira sampuro zvakakwana kunobatsira zvikuru pakushanda zvakanaka kwe centrifugation, kunyanya kana uchishanda nemaitiro asina kunaka. Kunyora nemazvo uye kubata sampuro, kubvisa tsvina, uye kushandisa ma buffers akakodzera kana michina yekudzivirira kuchaita kuti pave nemigumisiro yakavimbika uye inodzokororwa.

5. Ita Kumhanya Kwakawanda kana Kutenderera Pamberi: Kana zvichikwanisika, kuita kumhanya kwakawanda kana kuita kutenderera kwenguva pfupi nekumhanya kwakakwirira kunogona kubatsira kukunda zvipingamupinyi zvine chekuita ne centrifugation ine kumhanya kwakaderera. Danho rekutanga iri rinogona kuisa sampuli muchikamu chekutanga, zvichiita kuti zvive nyore kuparadzanisa sampuli panguva yekushanda kwekukurumidza kwakaderera.

Kuve nechokwadi chekururama uye kuchengetedzeka mukushandiswa kwemhepo mumhepo ine kumhanya kwakaderera:

Paunenge uchishandisa centrifuge panzvimbo dzakaderera, zvakakosha kuchengetedza kururama uye kuchengetedzeka. Heano mamwe mazano akakosha:

1. Kuenzanisa maSwing-Bucket Rotors: Kuenzanisa rotor ye centrifuge nekuparadzira machubhu emuenzaniso zvakaenzana munzvimbo dzakatarisana kunobatsira kuderedza kudedera uye kudzivirira kukuvara kwemidziyo panguva yekushanda nekukurumidza.

2. Tarisa Kucherwa Kwemvura Nekupatsanurwa: Gara uchitarisa sampuro panguva yekuchenesa mvura kuti uongorore kufambira mberi kwecherwa uye kupatsanurwa kwayo. Danho iri rinobatsira kuona kana kugadzirisa kuchidikanwa kuti uwane mhedzisiro yakanaka.

3. Tevedzera Maitiro Akanaka Emurabhoritari (GLP): Kutevedzera gwara reGLP, sekupfeka michina yekudzivirira munhu yakakodzera (PPE), kushandisa machubhu seal akakodzera, uye kunyora mamakisi nemazvo, kunoita kuti kuyedza kuchengetedzeke uye kuchengetedza kuvimbika kwekuyedza.

4. Shanda pamwe chete neNyanzvi: Kana uchishanda nemuenzaniso wausingazive kana kusangana nematambudziko ane chekuita ne centrifugation inokurumidza kuderera, nyanzvi dzinopa mazano mumunda uyu, dzakadai sevagadziri ve centrifuge kana mainjiniya vane ruzivo, vanogona kupa ruzivo rwakakosha nenhungamiro.

Mhedziso:

Kuisa centrifugation pamichina yakaderera kunoda kugadziriswa kwakanyatsofungwa kuti ukunde matambudziko uye uwane mhedzisiro yakavimbika. Nekusarudza marotor akakodzera nokungwarira, kugadzirisa nguva netembiricha, kushandisa zvinosimudzira centrifugation, kugadzirisa kugadzirira kwemuenzaniso, uye kutevedzera mitemo yekuchengetedza, vaongorori vanogona kufamba ne centrifugation nekukurumidza uku vachichengetedza kururama, kushanda zvakanaka, uye kuchengetedzeka.