Sentrifuge e arola likarolo joang

Ho Centrifugation: Mokhoa o Matla oa ho Arohanya Likarolo

Lefapheng la saense le theknoloji, mokhoa o mong o fetotseng tshebetso ya ho arola dikarolo ke ho centrifugation. Sesebelisoa sena se matla se fumane ditshebediso tsa sona diindastering tse fapaneng, ho tloha tlhokomelong ya bophelo ho ya k'hemistring le saenseng ya tikoloho. Ka bokgoni ba sona ba ho arola metswako ka potlako ho latela bongata, ho centrifugation e eme e le mokgwa wa bohlokwa. Empa centrifuge e phethahatsa mosebetsi ona jwang? Sehloohong sena, re tla hlahloba tshebetso ya centrifuge, melaomotheo e ka mora ho centrifugation, le ditshebediso tsa yona tse ikgethang karohanong ya dikarolo.

I. Metheo ea ho Centrifugation

Ho utloisisa hore na centrifuge e arola likarolo joang, re hloka ho utloisisa melao-motheo ea motheo pele. Bohareng ba eona, centrifuge e sebelisa matla a centrifugal, e leng matla a bohata a simolohang ho potoloha ha ntho, ho arola lintho ka potlako motsoakong ho latela bongata ba tsona. Khopolo ke ho beha motsoako lebelo le phahameng la ho potoloha, e leng se etsang hore likarolo tse teteaneng li tlohe hole le axis ea potoloho, ha tse sa teteaneng haholo li ntse li le haufi le eona.

II. Mochini oa Centrifuge: Mokhoa oa Karohano

Mochini oa centrifuge ka booona o na le likarolo tse 'maloa tsa bohlokoa. 'Mele o moholo o na le rotor, e khonang ho tšoara mefuta e fapaneng ea lipeipi kapa lijana tsa sampole. Ho rotor ho na le enjene e fanang ka matla a ho potoloha a hlokahalang, e nolofalletsang karohano ea lebelo le phahameng. Ho boloka botsitso nakong ea ts'ebetso, rotor e potoloha holim'a sete ea li-bearings. Ho phaella moo, mehato ea ts'ireletso e kang sekoahelo kapa sekoahelo e kenngoa tšebetsong ho thibela likotsi kapa tšilafalo ea sampole.

III. Mekhoa ea ho Centrifugation

Ho na le mekhoa e fapaneng ea ho centrifugation ho fihlela litlhoko tse itseng tsa karohano. Mekhoa e 'meli e tloaelehileng ke ho centrifugation e fapaneng le ho centrifugation ea gradient ea density. A re e hlahlobeng haholoanyane.

1. Phapang ea Centrifugation

Ho arola likarolo ka tsela e fapaneng hangata ke mohato oa pele karohanong ea likarolo. Sepheo sa eona ke ho arola likaroloana tse kholo, joalo ka lisele kapa li-organelle, ho tse nyane. Ka ho sebelisa matla a fapaneng a centrifugal ka lebelo le bolelele bo fapaneng, likarolo li aroloa ka tatellano ho latela boholo le bongata ba tsona. Ka mokhoa ona o tsamaeang ka mehato, lintho tse khahlisang li ka aroloa butle-butle, e leng se etsang hore ho arola likarolo ka tsela e fapaneng ho sebetse hantle le ho tenyetseha.

2. Sekhahla sa Gradient sa Botenya

Ho potoloha ha gradient ka bongata ho latela molao-motheo oa hore likarolo tse fapaneng li na le boholo bo fapaneng ba ho phaphamala. Ho kenyelletsa ho theha gradient ea bongata ho sebelisoa litharollo tsa boima bo fapaneng, joalo ka sucrose kapa cesium chloride. Sampole e kenngoa holimo ho gradient, 'me ha e ntse e potoloha ka har'a centrifuge, likarolo tse teteaneng lia falla ka har'a gradient, qetellong li theha lihlopha tse fapaneng. Mokhoa ona o thusa haholo bakeng sa ho arola limolek'hule tsa baeloji joalo ka DNA kapa RNA, le bakeng sa ho arola likaroloana tsa lisele.

IV. Litšebeliso tsa Bohlokoa tsa Centrifugation

Ho sebelisa centrifugation ho bapala karolo ea bohlokoa masimong a fapaneng ka lebaka la ho tenyetseha ha eona le katleho ea eona. Tse ling tsa lits'ebetso tse hlokomelehang ke tsena:

1. Tlhahlobo ea Bongaka le ea Bongaka

Bongaka le tlhahlobong, ho thiba lisele tsa mali ho bohlokoa haholo bakeng sa ho arola likarolo tsa mali tse kang lisele tse khubelu tsa mali, lisele tse tšoeu tsa mali le plasma. Sena se nolofalletsa ho ithuta mafu a fapaneng, ho hlahloba tšoaetso, le ho hlahloba mafu a liphatsa tsa lefutso. Ho feta moo, ho thiba lisele tsa mali ho thusa ho arola serum, e nang le limolek'hule tsa bohlokoa le li-enzyme tse sebelisoang litekong tsa tlhahlobo.

2. Indasteri ea Meriana

Indastering ea meriana, ho thiba lisele ho sebelisoa ho hloekisa le ho kopanya limolek'hule tsa baeloji tse kang liprotheine le li-antibodies. Ka ho arola likarolo tsena tsa bohlokoa ho tsoa motswakong, bo-rasaense ba ka hlahisa meriana e sireletsehileng le e sebetsang haholoanyane. Ho feta moo, ho thiba lisele ho thusa ho tlosa litšila le ho hloekisa metsoako e sebetsang ea meriana (API).

3. Lithuto tsa Tikoloho

Ho sebelisa centrifugation ho fumana lits'ebetso lithutong tsa tikoloho ho sekaseka le ho beha leihlo litšila tse ka metsing, mobung kapa moeeng. Ka karohano ea centrifugation, lintho tse nyenyane le lintho tse tiileng tse emisitsoeng li ka aroloa, e leng se thusang ho khetholla le ho lekanya lintho tse kotsi. Tsebo ena e thusa ho theha maano a sebetsang a taolo ea tšilafalo le tokiso ea tikoloho.

4. Biotechnology ea Liindasteri

Ho centrifugation ho bapala karolo ea bohlokoa theknolojing ea baeloji ea indasteri, moo ho arola le ho tsepamisa limolek'hule tse lakatsehang. Litšebeliso li kenyelletsa tlhahiso ea li-enzyme, ho ntša metabolite ea bohlokoa, le ho hloekisa biodiesel, ho bolela tse 'maloa feela. Ka ho sebelisa matla a ho centrifugation, lits'ebetso tsa indasteri li ka ba le katleho e kholo, tsa theko e tlase, le tse mosa tikolohong.

5. Indasteri ea Lijo le Lino

Indastering ea lijo le lino, ho sebelisa centrifugation ho sebelisoa bakeng sa ho hlakisa, ho arola le ho hloekisa lino tse metsi. Mohlala, e thusa ho hlahisa lero la litholoana le hlakileng, ho arola lintho tse tiileng lebeseng kapa biring, le ho hloekisa oli e jeoang. Mekhoa ena e netefatsa lihlahisoa tsa boleng bo holimo le ho ntlafatsa nako ea tsona ea ho boloka.

Qetellong, ho centrifugation ke mokhoa o ikhethang o fetotseng karohano ea likarolo. Bokhoni ba eona ba ho arola lintho ho latela bongata bo fumane lits'ebetso masimong a mangata, moo e thusang lipatlisisong tsa saense, tlhahlobo ea mafu, nts'etsopele ea meriana, lithuto tsa tikoloho, theknoloji ea baeloji ea indasteri le ts'ebetso ea lijo. Ebang ke ho centrifugation e fapaneng kapa ho centrifugation ea gradient ea bongata, mechine le mekhoa e ka morao ho centrifugation ntle ho pelaelo e bapetse karolo ea bohlokoa ho ntlafatseng kutloisiso ea saense le bokhoni ba theknoloji.