centrifuge e arola metsoako ka likarolo tse fapaneng

Sentrifuge: Sesebelisoa se Matla sa ho Arola Metswako ka Dikarolo

Selelekela

Li-centrifuge ke lisebelisoa tsa bohlokoa tsa laboratori tse bapalang karolo ea bohlokoa ho aroleng metsoako ho latela bongata bo fapaneng ba likarolo tsa tsona. Sesebelisoa sena se matla se sebelisoa haholo liindastering tse fapaneng, ho kenyeletsoa tlhokomelo ea bophelo bo botle, meriana, k'hemistri le mahlale a tikoloho. Sehloohong sena, re tla hlahloba ts'ebetso le ts'ebeliso ea li-centrifuge, re totobatsa bohlokoa ba tsona lipatlisisong, tlhahlobong le lits'ebetsong tsa tlhahiso.

I. Ho utloisisa Centrifugation

Ho centrifugation ke mokhoa oo ka ona metswako e arolwang ka dikarolo tsa yona ho latela bongata ba tsona bo fapaneng. E sebedisa molaomotheo wa matla a centrifugal, a hlahiswang ka ho potoloha sampole ka lebelo le phahameng. Ka lebaka leo, dikarolwana tse teteaneng kapa dikarolo di ya tlase ho tube ya centrifuge, di etsa dikarolo tse fapaneng.

II. Likarolo le Mokhoa oa ho Sebetsa

Sentifuge e na le dikarolo tse fapaneng, ho kenyeletsoa rotor, diphaephe tsa centrifuge, le enjene. Rotor e tshwara diphaephe tsa sampole ka dikhutlo tse tsitsitseng ha e ntse e potoloha ka lebelo le phahameng. Diphaephe tsa centrifuge di na le motswako o lokelang ho arolwa, e leng se dumellang ho senyeha ha dikarolo ka katleho.

Mokhoa oa ho sebetsa oa centrifuge o kenyelletsa mehato e 'maloa. Taba ea pele, lipeipi tsa sampole li tlatsoa ka motsoako ebe li kenngoa ka hloko ka har'a rotor. Centrifuge e beoa lebelo le nako e lakatsehang. Ha rotor e qala ho potoloha, matla a centrifugal a hula likarolo tse teteaneng ho ea tlase ho lipeipi, a etsa mekhahlelo e fapaneng.

III. Mefuta ea Li-Centrifuge

Li-centrifuge li fumaneha ka mefuta e fapaneng, e 'ngoe le e 'ngoe e sebeletsa merero e fapaneng ho latela litlhoko tse itseng tsa ts'ebeliso. Mefuta e tloaelehileng e kenyelletsa:

1. Li-Centrifuge tsa Benchtop: Li-centrifuge tsena tse nyane li etselitsoe mosebetsi o tloaelehileng oa laboratori. Hangata li sebelisoa bakeng sa meqolo e menyenyane ea sampole, joalo ka litleliniking tsa bongaka, lilaboratoring tsa lipatlisiso le liunivesithing.

2. Microcentrifuges: Di-centrifuge tsena di etseditswe ka ho kgetheha ho sebetsana le dibolumo tse nyane, hangata di tloha ho 0.2 mL ho isa ho 2 mL. Di sebediswa hangata baelojing ya dimolek'hule le dipatlisisong tsa DNA.

3. Li-Ultracentrifuge: Li-Ultracentrifuge ke li-centrifuge tse matla ka ho fetisisa le tse feto-fetohang tse fumanehang. Li ka fihlella lebelo le phahameng haholo, li nolofalletsa karohano ea metsoako e rarahaneng, joalo ka livaerase, liprotheine le mafura. Hangata li-ultracentrifuge li sebelisoa lipatlisisong tse tsoetseng pele le tlhahisong ea ente.

4. Li-Centrifuge tsa ho Lokisetsa: Li-centrifuge tsena li sebelisetsoa merero e meholo ea tlhahiso, joalo ka ho arola likarolo tse itseng kapa ho hloekisa lintho ka bongata. Li sebelisoa haholo indastering ea meriana le ea baeloji.

IV. Dikopo tsa Tlhokomelo ea Bophelo le Tlhahlobo

Li-centrifuge ke lisebelisoa tsa bohlokoa indastering ea tlhokomelo ea bophelo le tlhahlobo. Li sebelisoa haholo lits'ebetsong tse fapaneng tsa bongaka, ho kenyeletsoa liteko tsa mali, tlhahlobo ea moroto, le karohano ea plasma kapa serum ho tsoa lisampoleng tsa mali kaofela. Ka ho potoloha lisampole tsa mali ka har'a lipeipi tsa centrifuge, litsebi tsa tlhokomelo ea bophelo li ka arola likarolo tsa sele ho tsoa karolong ea metsi, e leng se lumellang tlhahlobo e nepahetseng le tlhahlobo ea mafu a fapaneng.

V. Bohlokoa Patlisisong le Lithutong tsa Saense

Li-Centrifuge li fetotse lipatlisiso tsa saense ka ho fana ka mokhoa oa ho arola metsoako e rarahaneng ka likarolo tsa eona ka bomong. Li nolofalletsa bo-rasaense ho ithuta likaroloana kapa lintho tse itseng, ho sekaseka thepa ea tsona, le ho etsa liteko tse ling. Centrifuge e sebelisoa haholo ho biochemistry, baeloji ea limolek'hule, virology, le mafapheng a mang a mangata a saense, e leng se tlatsetsang haholo tsoelo-peleng mafapheng ana.

VI. Litšebeliso tsa Tikoloho

Li-centrifuge li boetse li fumana ts'ebeliso ea bohlokoa mahlaleng a tikoloho. Li bapala karolo ea bohlokoa lifemeng tsa kalafo ea metsi a litšila, moo li arolang litšila tse tiileng le seretse ho tsoa metsing, li thusa lits'ebetsong tse sebetsang hantle tsa tlhoekiso. Ho feta moo, li-centrifuge li sebelisoa ho ithuteng sebopeho sa mobu, ho khetholla litšila kapa litšila, le ho sekaseka lisampole tsa tikoloho.

Qetello

Li-centrifuge ke lisebelisoa tse makatsang tse lumellang karohano ea metsoako likarolong tsa eona ka bonngoe ho latela phapang ea bongata. Ho tloha tlhahlobong ea tlhokomelo ea bophelo bo botle ho ea lipatlisisong tsa saense le lithutong tsa tikoloho, lisebelisoa tsena tse matla li na le mefuta e mengata ea lits'ebetso. Ha theknoloji e ntse e tsoela pele, li-centrifuge li ntse li tsoela pele ho fetoha, li nolofalletsa bafuputsi le liindasteri ho hlahloba menyetla e mecha le ho etsa litšibollo tse ncha.