nzira yekuparadzanisa serum kubva kuropa pasina centrifuge

Maitiro Ekuparadzanisa Serum neRopa Pasina Centrifuge

Nhanganyaya

Kuparadzanisa serum kubva muropa inzira yakakosha mukushandiswa kwakasiyana-siyana kwekurapa nesainzi. Zvisinei, nzira dzechinyakare dzinowanzoda michina yakakosha senge centrifuges, zvichiita kuti maitiro acho asasvike kana kuti ave akaoma mune dzimwe nzvimbo. Muchinyorwa chino, tichaongorora dzimwe nzira dzekuparadzanisa serum kubva muropa pasina kushandisa centrifuge. Maitiro aya anopa nyore, kushanda zvakanaka, uye kushanda zvakanaka, zvichiita kuti akwanise kushandiswa mumamiriro akasiyana-siyana.

1. Nzira yekusefa: Kuparadzanisa Serum Uchishandisa Mafirita

Imwe yenzira dzinoshanda dzekuparadzanisa serum kubva kuropa pasina centrifuge ndeyekushandisa mafirita. Nzira iyi inotsamira pakusiyana kwehukuru pakati pemasero neserum kuti ive nechokwadi chekuparadzaniswa kunobudirira. Heino gwara rekuita nhanho nhanho:

a. Unganidza zvinhu zvinodiwa:

- Bepa rekusefa kana membrane

- Funnel

- Pipette kana dropper

- Machubhu ekuyedza

b. Gadzirira gadziriro yesefa:

- Peta bepa rekusefa nenzira inoenderana nefunnel kana kushandisa membrane yekusefa yakachekwa kare.

- Isa jira pamusoro pechubhu yekuyedza.

c. Isai ropa:

- Uchishandisa pipette kana dropper, isa ropa racho nokungwarira pabepa rekusefa kana membrane. Ita shuwa kuti waisa huwandu hwakakwana kuti uvhare pamusoro pese.

d. Bvumira kusefa kwegiravhiti:

- Siya zvakarongeka uye rega serum ifambe nepapepa kana membrane ichipinda muchubhu yekuyedza. Izvi zvinogona kutora nguva, saka mwoyo murefu unokosha.

e. Kuunganidza serum yakaparadzaniswa:

- Kana kusefa kwapera, bvisa chubhu yekuyedza nokungwarira, iyo ikozvino ine seramu yakaparadzaniswa.

2. Nzira yekubvisa madhaka: Rega Simba reGravity Riparadzanise Serum

Kubvisa tsvina muropa inzira imwe iri nyore inoshandisa simba rinokwevera pasi kuparadzanisa ropa kubva muropa. Nzira iyi inoshandisa musiyano wehuwandu hwemasero eropa neropa. Tevedzera matanho aya kuparadzanisa ropa uchishandisa nzira yekuparadza tsvina:

a. Kutora ropa:

- Uchishandisa sirinji kana nzira yakakodzera yekuunganidza ropa, bvisa ropa raunoda mumudziyo wakachena.

b. Rega muenzaniso umire:

- Rega ropa rigare risina kukanganiswa kwenguva yakatarwa, kazhinji maminetsi makumi matatu kusvika paawa imwe chete. Nguva iyi yekuzorora inobvumira kuti ropa rive netsvina.

c. Tarisa kupatsanurwa:

- Mushure menguva yekuzorora, uchaona zvidimbu zviviri zvakasiyana mumudziyo. Chidimbu chepamusoro chinomiririra seramu yakaparadzaniswa, nepo chikamu chepasi chiine masero eropa.

d. Bvisa serum nokungwarira:

- Uchishandisa pipette kana kubvisa tsvina zvakanyatsonaka, bvisa chikamu chepamusoro cheromu pasina kukanganisa chikamu chezasi.

3. Midziyo yeMicrofluidic: Lab-on-a-chip yekuparadzanisa serum

Kufambira mberi mu tekinoroji ye microfluidic kwakagadzira nzira yemidziyo ye compact lab-on-a-chip (LOC) inogona kuparadzanisa serum kubva muropa zvinobudirira. Midziyo iyi inoshandisa nzira dze microchannels uye nzira dzakasiyana-siyana dzekuparadzanisa kuti iwane mhedzisiro yekuparadzanisa nekukurumidza uye yakavimbika. Heino pfupiso yemaitiro acho:

a. Isa muenzaniso:

- Uchishandisa pipette, isa ropa mumudziyo we microfluidic kana cartridge yakagadzirirwa kupatsanurwa kweserum.

b. Isa kumanikidzwa kana kushanda kwe capillary:

- Zvichienderana nemudziyo, shandisa simba kana kushandisa capillary action kuti utange kupatsanurana.

c. Tarisa kupatsanurwa kwechip:

- Chip ye microfluidic ichave ine nzira kana membranes dzakatarwa dzinobatsira kupatsanurwa kweserum kubva kumasero eropa. Tarisa kuyerera nekuparadzaniswa kuburikidza nemaikorosikopu kana sisitimu yakakodzera yekuona.

d. Kuunganidza seramu yakaparadzaniswa:

- Panzvimbo yakatarwa yekubuda, tora serum yakaparadzaniswa kuti iongororwe kana kuchengetwa.

4. Nzira yeMakemikoro: Kushandisa Zvinoshandiswa Pakuparadzanisa

Nzira dzemakemikari dzinopa imwe nzira yekuparadzanisa serum kubva kuropa pasina centrifuge. Nzira idzi dzinoshandisa nzira dzekuparadzanisa kana ma reagents anobatsira kukonzera kupatsanurwa zvichibva pane zvimwe zvinhu. Heino tsananguro yekushanda kwenzira iyi:

a. Wedzera chinhu chinoparadzanisa:

- Zvichibva pane chinhu chakasarudzwa chekuparadzanisa ropa, wedzera huwandu hwakakodzera kana huwandu hwakakodzera muropa.

b. Bvumira nguva yekuparadzana:

- Ipai muenzaniso nguva yakakwana yekubata nemushonga wekuparadzanisa, zvichiita kuti kupatsanurwa kunodiwa kuitike.

c. Ita danho rekuparadzanisa:

- Isa danho rekuparadzanisa, senge centrifugation nekumhanya kwakaderera pane kunowanzo dikanwa, kuti upedzise maitiro ekuparadzanisa.

d. Kuunganidza seramu yakaparadzaniswa:

- Mushure medanho rekupatsanurana, unganidzai serum yakapatsanurwa nokungwarira, muchiichengeta yakaparadzana nezvimwe zvikamu zveropa zvasara.

Mhedziso

Kunyange hazvo ma "centrifuge" achiwanzo shandiswa kupatsanura serum kubva muropa, kune dzimwe nzira dzinoita kuti zvive nyore uye zvive nyore, kunyanya munzvimbo dzisina zviwanikwa zvakawanda. Nzira yekusefa, nzira yekusefa, michina ye microfluidic, uye nzira dzemakemikari zvese zvinopa sarudzo dzinoshanda dzekuparadzanisa serum pasina "centrifuge". Ingave iri mukiriniki, tsvakiridzo, kana nzvimbo yemunda, nzira idzi dzinoita kuti serum ibudirire kupatsanurwa kuti iongororwe uye iongororwe.