Me pēhea te wehe i te toto mai i te toto me te kore he centrifuge
Me pēhea te wehewehe i te toto mai i te toto me te kore he centrifuge
Kupu Whakataki
He tukanga nui te wehewehe i te toto mai i te toto i roto i ngā mahi hauora me ngā mahi pūtaiao. Heoi, he maha ngā wā ka hiahiatia he taputapu motuhake pērā i ngā mīhini whakapūtā, ā, ka uaua te tukanga i ētahi wāhi. I roto i tēnei tuhinga, ka tūhuratia e mātou ētahi atu tikanga hei wehewehe i te toto mai i te toto me te kore e whakamahi i te mīhini whakapūtā. He māmā noa iho, he pai te mahi, he iti hoki te utu o ēnei tikanga, ā, he pai mō ngā āhuatanga rerekē.
1. Tikanga Tātari: Te Wehewehe i te Huka mā te Whakamahi i ngā Tātari
Ko tētahi o ngā tikanga whai hua hei wehewehe i te toto mai i te toto, me te kore he centrifuge, ko te whakamahi i ngā tātari. Ka whakawhirinaki tēnei tikanga ki te rerekētanga rahi i waenga i ngā pūtau me te toto hei whakarite kia angitu te wehenga. Anei tētahi aratohu taahiraa-i-te-taahiraa mō te mahi i tēnei:
a. Kohikohia ngā rauemi e tika ana:
- Pepa tātari, kiriuhi rānei
- Kōrere
- Paipa, pata rānei
- Ngā ngongo whakamātautau
b. Whakaritea te whakatakotoranga tātari:
- Whakapikohia te pepa tātari kia rite ki te āhua o te hāneanea, whakamahia rānei he kiriuhi tātari kua tapahia kē.
- Whakatakotoria te kōrere ki runga i te ngongo whakamātautau.
c. Tāpirihia te tauira toto:
- Mā te whakamahi i tētahi pipette, i tētahi pata rānei, āta pania te tauira toto ki runga i te pepa tātari, i te kiriuhi rānei. Kia tino pania te nui hei taupoki i te mata katoa.
d. Tukua te tātari ā-papatipu:
- Waiho te whakaritenga kia kaua e whakakorikoria, ā, tukua te hinu kia tātari mā roto i te pepa, i te kiriuhi rānei ki roto i te ngongo whakamātautau. Tērā pea ka roa te wā mō tēnei tukanga, nō reira he mea nui te manawanui.
e. Kohia te serum kua wehea:
- Kia oti te tātari, tangohia marietia te ngongo whakamātautau, kei roto nei te toto kua wehea.
2. Tikanga Whakatakotoranga: Tukua te Pūmua kia Wehe i te Huka
Ko te whakapūpū tētahi atu tikanga māmā e whakamahi ana i te kaha ā-papa hei wehe i te toto mai i te toto. Ka whakamahia e tēnei tikanga te rerekētanga mātotoru i waenga i te toto me ngā pūtau toto. Whāia ēnei mahi hei wehe i te toto mā te whakamahi i te tikanga whakapūpū:
a. Kohia te tauira toto:
- Mā te whakamahi i tētahi ngira, i tētahi tikanga kohikohi rānei e tika ana, tangohia te tauira toto e hiahiatia ana ki roto i tētahi ipu ma.
b. Tukua te tauira kia okioki:
- Tukua te tauira toto kia noho noa, kaua e whakakorikoria mō tētahi wā kua whakaritea, tata ki te 30 meneti ki te haora. Mā tēnei wā okioki ka taea te whakatakoto i te wai.
c. Mātakihia te wehenga:
- I muri i te wā okiokinga, ka kite koe i ngā paparanga motuhake e rua e puta mai ana i roto i te ipu. Ko te paparanga o runga e tohu ana i te toto kua wehea, ko te paparanga o raro ia kei roto ngā pūtau toto.
d. Tangohia marietia te serum:
- Mā te whakamahi i tētahi pipette, i tētahi ringihanga āta rānei, tangohia te paparanga o runga o te serum, kaua e whakararuraru i te paparanga o raro.
3. Ngā Pūrere Maikorowai: Ngā Pūrere Taiwhanga-i-runga-i-te-maramara mō te Wehenga o te Huka
Nā ngā whanaketanga o te hangarau moroiti wai kua whakawātea i te ara mō ngā taputapu taiwhanga-i-runga-i-te-maramara (LOC) kiato ka taea te wehewehe pai i te toto mai i te toto. Ka whakamahia e ēnei taputapu ngā awa moroiti me ngā tikanga wehewehe rerekē hei whakatutuki i ngā hua wehewehe tere me te pono. Anei tētahi tirohanga whānui o te tukanga:
a. Utaina te tauira:
- Mā te whakamahi i tētahi pipette, whakauruhia te tauira toto ki roto i te taputapu moroiti wai, i te kātereti rānei i hangaia mō te wehenga toto.
b. Whakamahia te pēhanga, te mahi ā-pūwero rānei:
- I runga anō i te taputapu, pēhia, whakamahia rānei te mahi ā-pūwero hei whakaoho i te tukanga wehenga.
c. Tirohia te wehenga i runga i te maramara:
- Kei roto i te maramara moroiti wai ngā awa motuhake, ngā kiriuhi rānei, hei āwhina i te wehenga o te toto mai i ngā pūtau toto. Mātakihia te rere me te wehenga mā te karuārai, mā te pūnaha kimi rānei e tika ana.
d. Kohia te serum kua wehea:
- Ki te putanga kua tohua, kohia te serum kua wehea hei tātaritanga anō, hei penapena rānei.
4. Tikanga Matū: Te Whakamahi i ngā Āhua Wehewehe
He huarahi kē atu anō kei roto i ngā tikanga matū hei wehe i te toto mai i te toto, me te kore whakamahi i te centrifuge. E whakawhirinaki ana ēnei tikanga ki te whakamahi i ngā kaihoko wehewehe, i ngā tauhohenga rānei hei āwhina i te wehenga i runga i ngā āhuatanga motuhake. Anei tētahi whakarāpopototanga whānui o te mahi a tēnei tikanga:
a. Tāpirihia te kaihoko wehewehe:
- I runga i te kaihoko wehewehe kua whiriwhiria, tāpirihia te rahinga, te kukū rānei e tika ana ki te tauira toto.
b. Tukua he wā mō te wehenga:
- Hoatu he wā e tika ana ki te tauira kia tauhohe ki te kaihoko wehewehe, kia taea ai te wehenga e tika ana.
c. Whakahaerehia he taahiraa wehewehe:
- Whakamahia he taahiraa wehenga, pērā i te centrifugation i te tere iti iho i te tere e hiahiatia ana, hei whakaoti i te tukanga wehenga.
d. Kohia te serum kua wehea:
- Whai muri i te mahi wehenga, kohia marietia te serum kua wehea, kia wehea mai i ngā wāhanga toto e toe ana.
Whakamutunga
Ahakoa e whakamahia whānuitia ana ngā mīhini whakapūtā hei wehe i te toto mai i te toto, kei reira ētahi atu tikanga e māmā ake ai, e whai hua ai hoki, inā koa i roto i ngā wāhi iti nei ngā rauemi. Ko te tikanga tātari, te tikanga whakatō, ngā taputapu moroiti wai, me ngā tikanga matū katoa e tuku ana i ngā kōwhiringa whai hua hei whakatutuki i te wehenga toto me te kore whakamahi i te mīhini whakapūtā. Ahakoa i roto i te wāhi haumanu, rangahau, mara rānei, mā ēnei tikanga ka taea te wehe angitu i te toto mō ngā tātaritanga me ngā take tātaritanga anō.
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