mudziyo we centrifuge unogona kuparadzanisa munyu nemvura here?

Ko Centrifuge Inogona Kuparadzanisa Munyu Nemvura Here?

Nhanganyaya:

Kupinza mvura mu "centrifugation" inzira inoshandiswa zvakanyanya muzvikamu zvakasiyana-siyana, kubva pakutsvagisa kwesainzi kusvika kumashandisirwo emaindasitiri. Mubvunzo unowanzo bvunzwa ndewekuti "centrifuge" inogona here kuparadzanisa munyu nemvura zvinobudirira. Muchinyorwa chino, tichaongorora sainzi ye "centrifugation" uye toongorora kuti ingashandiswa sei kuparadzanisa munyu nemvura. Kubva pakunzwisisa misimboti yeiyi nzira kusvika pakuongorora miganhu yayo, chinangwa chedu ndechekupa ruzivo rwakakwana.

Sainzi iri shure kweCentrifugation:

1. Nheyo yeSimba reCentrifugal

Kana musanganiswa ukaiswa mu centrifuge, unoiswa pasi pesimba re centrifugal. Simba iri rinoita kuti zvikamu zvakawandisa zvifambe zvichienda kumadziro ekunze echubhu ye centrifuge, zvichiita kuti pave ne pellet, nepo zvikamu zvisina kusimba zvakanyanya, zvakaita semvura, zvichiramba zviri pedyo nepakati.

2. Kuparadzaniswa kweMunyu neMvura

Munyu unonyungudika nyore nyore mumvura, zvichiita maion anopararira zvakaenzana mumvura yese. Nekuti maion emunyu anopararira uye haaunganidzwe zvakanyanya, haakanganisike nesimba re centrifugal. Nekuda kweizvozvo, kungoshandisa centrifuge kutenderedza musanganiswa wemunyu nemvura hakuzoiti kuti zviparadzane.

Basa reDensity-Gradient Centrifugation:

Kuti muparadzanise munyu nemvura muchishandisa centrifuge, imwe nzira inonzi density-gradient centrifugation inogona kushandiswa. Nzira iyi inoshandisa kugadzira density gradient mukati mechubhu yecentrifuge.

3. Kugadzirwa kweDensity Gradient

Kuwanda kwehuwandu hwemvura kunogona kugadzirwa nekuwedzera zvinhu zvakaita sesucrose kana cesium chloride mumushonga. Sezvo mushonga wacho uchiiswa pakati, zvinhu zvinoumba kuyera uko huwandu hwemvura hunowedzera kana kuderera zvishoma nezvishoma pakureba kwechubhu.

4. Maitiro Ekuparadzanisa

Kana musanganiswa wacho waiswa muchubhu ine density-gradient, zvidimbu zvemunyu zvinorema zvinofamba zvichienda kunzvimbo ine density yakanyanya, ukuwo mamorekuru emvura achiramba ari pedyo nepakati. Izvi zvinobvumira kupatsanurwa kwemunyu nemvura zvishoma.

Miganhu yeCentrifugation:

5. Kushanda Nesimba Uye Kuchena

Kunyange hazvo kupatsanurwa kwemunyu nemvura kunogona kuita kuti mvura iparadzwe, zvakakosha kuziva kuti kupatsanurwa kwemunyu hakugoneki nguva dzose. Munyu mumwe unogona kuramba uri muchikamu chemvura, zvichikonzera kuti mvura isacheneswe zvakakwana.

6. Zvimwe Zvisina Kuchena

Kupinza zvinhu mu "centrifugation" kunoshanda zvakanyanya mukuparadzanisa zvinhu zvichienderana nekuwanda kwazvo. Zvisinei, hakubvisi zvimwe zvinhu zvisina kuchena, zvakaita semagasi akanyungudutswa, makemikari emuchadenga, kana zvidimbu zvakanyungudutswa zvinogona kunge zviri mumusanganiswa wemvura nemunyu.

7. Zvinodiwa Nesimba

Kupinza simba mu centrifugation inzira inoda simba rakawanda. Kuti kupatsanurwa kubudirire, nguva refu yekutenderera nekukurumidza inodiwa, izvo zvinogona kuwedzera kushandiswa kwesimba rose mukuita uku.

Mashandisirwo eCentrifugation:

8. Mashandisirwo Emaindasitiri

Kuchenesa simbi kunoshandiswa zvakanyanya mumaindasitiri akasiyana-siyana, anosanganisira mishonga, biotechnology, uye kugadzira chikafu. Inoshandiswa zvakanyanya pakuparadzanisa zvinhu zvakasimba kubva mumvura, pamwe nekuchenesa nekusanganisa zvinhu zvakasiyana-siyana.

9. Tsvagiridzo yeMolecular Biology

Mukutsvaga kwebiology yemamorekuru, centrifugation inoita basa guru mukuchenesa DNA, RNA, uye mapuroteni. Iyi nzira inogonesa masayendisiti kudzidza nekushandura mamorekuru aya kuti ashandiswe zvakasiyana-siyana, kusanganisira genetic engineering uye diagnostics.

Mhedziso:

Kupinza munyu mumvura, kunyangwe kusingakwanise zvakananga kuparadzanisa munyu wakachena nemvura, kunogona kuva nzira yakakosha mukubatsira maitiro ekuparadzanisa munyu. Nekushandisa density-gradient centrifugation, zvinokwanisika kuparadzanisa munyu nemvura zvishoma. Zvisinei, kuchenesa munyu zvakakwana hakugone kuitwa kuburikidza nenzira iyi chete, sezvo isingabvisi tsvina yese. Pasinei nemiganhu yayo, kupinza munyu mumvura kunoramba kuri chishandiso chinokosha mumashandisirwo akasiyana-siyana esainzi nemaindasitiri, zvichiita kuti zvinhu zvakasiyana-siyana zvikwanise kupatsanurwa nekucheneswa.