ka taea e te centrifuge te wehewehe i te tote mai i te wai
Ka taea e te centrifuge te wehe i te tote mai i te wai?
Kupu Whakataki:
He tikanga whānui te whakamahi i te centrifugation i roto i ngā momo mara, mai i te rangahau pūtaiao ki ngā tono ahumahi. Ko tētahi pātai e puta ake ana ko te kaha o te centrifuge ki te wehewehe i te tote mai i te wai. I roto i tēnei tuhinga, ka rukuhia e mātou te pūtaiao o te centrifugation, ā, ka tūhuratia me pēhea te whakamahi hei wehewehe i te tote mai i te wai. Mai i te mārama ki ngā mātāpono o tēnei tikanga ki te tirotiro i ōna herenga, ko tā mātou whāinga he whakarato i tētahi tirohanga whānui.
Te Pūtaiao kei muri i te Whakapūtanga:
1. Te Mātāpono o te Kaha Pūtahi
Ina whakanohoia he ranunga ki roto i te mīhini whakateretere, ka pāngia e te kaha whakateretere. Mā tēnei kaha ka neke ngā wāhanga matotoru ki te pakitara o waho o te ngongo whakateretere, ka hanga he pirepire, ko ngā wāhanga iti ake te matotoru, pērā i ngā wai, ka noho tata tonu ki te pokapū.
2. Te Wehenga o te Tote i te Wai
Ka tere te memeha o te tote ki roto i te wai, ka hanga he katote e horapa ōrite ana puta noa i te wai. Nā te mea ka marara ngā katote tote, ā, kāore e nui te whakakotahi, kāore rātou e pāngia e te kaha o te porotītaha. Nō reira, mā te whakamahi noa i te porotītaha hei hurihuri i te ranunga o te tote me te wai, e kore e wehea.
Te Tūranga o te Whakapūtanga Matū-Rōrahi:
Hei wehe i te tote mai i te wai mā te whakamahi i te centrifuge, ka taea te whakamahi i tētahi atu tikanga e kiia nei ko te density-gradient centrifugation. Ka whakawhirinaki tēnei tikanga ki te waihanga i tētahi gradient density i roto i te ngongo centrifuge.
3. Te Waihanga i te Rōnaki Matotoru
Ka taea te whakaputa i tētahi pikinga mātotoru mā te tāpiri i tētahi rauemi pērā i te sucrose, te cesium chloride rānei ki te wairewa. I te wā e hurihia ana te wairewa, ka hangaia he pikinga e te rauemi, ka piki haere, ka heke haere rānei te mātotoru i te roanga o te ngongo.
4. Tukanga Wehewehe
Ina whakatōngia te ranunga ki roto i tētahi ngongo whakarōpū-mātotoru, ka heke ngā matūriki tote mātotoru ki te rohe he mātotoru ake, ko ngā ngota wai ia e noho tata atu ana ki waenganui. Mā tēnei ka taea te wehe ā-wāhanga o te tote mai i te wai.
Ngā Here o te Whakapūtanga:
5. Te Whai Hua me te Parakore
Ahakoa ka taea e te whakamahi i te pūwero mātotoru-whakaheke te wehe i te tote mai i te wai, he mea nui kia mōhio kāore e taea te wehe katoa i ngā wā katoa. Tērā pea ka noho tonu ētahi tote ki roto i te wāhanga wai, ka hua ake he otinga kāore i tino parakore.
6. Ētahi atu poke
He tino whai hua te whakamahi i te pūwero hei wehewehe i ngā rauemi i runga i ō rātou matotoru. Heoi, kāore e whakakore i ētahi atu poke, pērā i ngā hau rewa, ngā matū waro, ngā matūriki iri rānei kei roto i te ranunga wai-tote.
7. Ngā Whakaritenga Pūngao
He tukanga e nui ana te kaha o te pūngao i te pūngao hurihuri. Hei whakatutuki i te wehenga whai hua, me roa te wā e hiahiatia ana te hurihuri tere-rahi, ka taea ai e tēnei te whakanui ake i te whakapaunga pūngao katoa o te tukanga.
Ngā tono o te centrifugation:
8. Ngā Whakamahinga Ahumahi
He whānuitia te whakamahinga o te centrifugation i roto i ngā ahumahi maha, tae atu ki ngā rongoā, te hangarau koiora, me te tukatuka kai. He mea noa te whakamahi mō te wehewehe i ngā mea totoka mai i ngā wai, tae atu ki te purenga me te kukū o ngā matū rerekē.
9. Rangahau Koiora Ngongo
I roto i te rangahau koiora ngota, he mea nui te mahi a te centrifugation ki te pure i te DNA, te RNA, me ngā pūmua. Mā tēnei tikanga ka taea e ngā kaipūtaiao te ako me te whakahaere i ēnei ngota mō ngā momo tono, tae atu ki te hangarau ira me te tātaritanga.
Whakatau:
Ahakoa kāore e taea te wehewehe tika i te tote parakore mai i te wai mā te whakamahi i te centrifugation, he tikanga nui pea tēnei hei āwhina i te tukanga wehewehe. Mā te whakamahi i te centrifugation mātotoru-whakaheke, ka taea te wehewehe ā-wāhanga i te tote mai i te wai. Heoi, kāore pea e taea te whakatutuki i te purenga katoa mā tēnei tikanga anake, nā te mea kāore e whakakorea ngā poke katoa. Ahakoa ōna herenga, he taputapu whai hua tonu te centrifugation i roto i ngā tono pūtaiao me ngā tono ahumahi, e āwhina ana i te wehenga me te purenga o te whānuitanga o ngā matū.
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