Kodi madzi oyeretsera madzi amathandiza bwanji kuti kulekanitsa madzi kukhale kogwira mtima?

Udindo wa Madzi Ozungulira Pakukulitsa Kugwira Ntchito Bwino kwa Kulekanitsa Madzi

Kugawa madzi m'maselo ndi njira yogawa madzi yomwe imagwiritsidwa ntchito kwambiri m'ma laboratories, m'mafakitale, komanso m'malo ofufuzira. Yapangidwa kuti ilekanitse zigawo za madzi osakaniza kutengera kuchuluka kwawo, kukula kwawo, ndi mawonekedwe awo. Kugwira ntchito bwino kwa njira yogawa madzi m'maselo kungawonjezeke kwambiri pogwiritsa ntchito madzi ogawa madzi m'maselo, omwe amagwira ntchito ngati njira yowongolera kulekanitsa zinthu zosiyanasiyana.

Kumvetsetsa Kugawa kwa Madzi ndi Kupatukana kwa Madzi

Kugawa mphamvu zamagetsi pogwiritsa ntchito centrifuge ndi njira yomwe imagwiritsa ntchito centrifuge kulekanitsa tinthu kuchokera ku yankho malinga ndi kukula kwake, mawonekedwe ake, kuchuluka kwake, kukhuthala kwa sing'anga, ndi liwiro la rotor. Madzi ogawa mphamvu akawonjezeredwa, amathandiza kuti pakhale kulekanitsidwa bwino mwa kupanga malo abwino kwambiri kuti tinthu tikhazikike bwino pa liwiro lawo labwino. Madzi ogawa mphamvu amagwiritsa ntchito ngati chotetezera tinthu pamene tikuyenda kudzera mu mphamvu ya centrifugal, zomwe zimathandiza kuti zigawozo zilekanitsidwe bwino.

Njira yolekanitsira madzi mu centrifuge imadalira mfundo za sedimentation ndi buoyant density. Sedimentation imatanthauza mphamvu yokoka yomwe imagwira ntchito pa tinthu tating'onoting'ono, zomwe zimapangitsa kuti tikhazikike pansi pa chubu cha centrifuge. Kumbali ina, buoyant density ndi mfundo yomwe imatsimikiza liwiro lomwe tinthu tating'onoting'ono timakwera kapena kumira mu centrifuge, kutengera kuchuluka kwawo poyerekeza ndi sing'anga. Kugwiritsa ntchito centrifuge liquid kumathandiza kukonza mfundozi kuti zithetse kulekanitsa bwino zigawo zamadzimadzi.

Mitundu ya Zamadzimadzi a Centrifuge

Pali mitundu yosiyanasiyana ya zakumwa zomwe zingagwiritsidwe ntchito pogawa madzi kuti ziwonjezere njira yolekanitsira madzi. Kusankha madzi ogawa madzi kumadalira mtundu wa chitsanzo chomwe chikukonzedwa komanso zofunikira za kulekanitsa. Mitundu yodziwika bwino ya madzi ogawa madzi ndi madzi, zosungunulira zachilengedwe, zolumikizira zamagetsi, ndi mayankho a buffer.

Madzi ndi madzi ogwiritsidwa ntchito kwambiri pogawa zinthu, makamaka pogawa zinthu. Amapereka njira yosavuta komanso yotsika mtengo yogawa zinthu kutengera kuchuluka ndi kukula kwake. Zosungunulira zachilengedwe, monga ethanol kapena isopropanol, zimagwiritsidwa ntchito pamene zofunikira zinazake za kusungunuka ndi kuchuluka kwake ziyenera kukwaniritsidwa. Zosungunulirazi zingathandize kuthetsa kulekanitsa bwino zinthu zachilengedwe kapena zitsanzo zamoyo.

Zipangizo zoyezera kuchuluka kwa tinthu, monga sucrose kapena cesium chloride, zimagwiritsidwa ntchito pamene pakufunika kulekanitsa bwino tinthu. Zipangizo zimenezi zimapangitsa kuti tinthu timeneti tisunthire ku mphamvu zawo zoyenda kuti tisiyanitse bwino. Komano, njira zoyezera kuchuluka kwa tinthu zimagwiritsidwa ntchito kusunga pH ndi mphamvu ya ionic ya chitsanzocho panthawi yoyezera kuchuluka kwa tinthu, komanso kupereka njira yoyenera yolekanitsira tinthu.

Kupititsa patsogolo Kugwiritsa Ntchito Madzi a Centrifuge

Kuonjezera madzi ozungulira (centrifuge liquid) kumawonjezera kwambiri magwiridwe antchito a njira yolekanitsira madzi m'njira zingapo. Choyamba, kumapereka njira yochepetsera kukangana pakati pa tinthu tating'onoting'ono ndi chubu cha centrifuge, zomwe zimathandiza kuti madzi asungunuke mosavuta komanso mwachangu. Izi zimapangitsa kuti zigawozo zigawikane mwachangu, zomwe zimachepetsa nthawi yonse yokonza.

Kachiwiri, kugwiritsa ntchito madzi ozungulira kumathandiza kupanga malo okhazikika kuti tinthu tikhazikike, zomwe zimapangitsa kuti zinthu zomwe zalekanitsidwa zikhale bwino komanso kuti zinthuzo zikhale zomveka bwino. Izi ndizofunikira kwambiri pochita zinthu zovuta kapena zitsanzo zomwe zili ndi tinthu tambirimbiri tosiyanasiyana. Madzi ozungulira amatsimikizira kuti kulekanitsidwako kumachitika popanda kusokoneza kapena kusakaniza zinthuzo.

Kuphatikiza apo, madzi ozungulira angathandizenso kuchotsa zodetsa ndi zinyalala kuchokera mu chitsanzo panthawi yolekanitsa. Mwa kupanga chotchinga pakati pa tinthu tating'onoting'ono ndi malo ozungulira, madzi ozungulira amathandiza kusunga chiyero cha zigawo zolekanitsidwa, zomwe zimapangitsa kuti pakhale zotsatira zabwino kwambiri.

Zinthu Zomwe Zimakhudza Kugwiritsa Ntchito Bwino kwa Kupatukana kwa Madzi

Zinthu zingapo zimakhudza magwiridwe antchito a kulekanitsa madzi mu centrifuge, ndipo kusankha madzi a centrifuge kumachita gawo lofunika kwambiri pakukonza zinthuzi. Kukhuthala kwa madzi a centrifuge ndikofunikira kuganizira, chifukwa kumakhudza kuyenda ndi kukhazikika kwa tinthu mkati mwa medium. Madzi otsika kukhuthala angathandize kuti madzi asungunuke mwachangu, pomwe madzi okwera kukhuthala angafunike kuti asiyanitse bwino.

Kuchulukana ndi kukhazikika kwa madzi a centrifuge kumakhudzanso magwiridwe antchito a kulekanitsa madzi. Kuchulukana koyenera bwino mkati mwa njira kungathandize kuti tinthu timeneti tipatuke bwino kutengera kuchulukana kwawo. Kukhazikika kwa madzi a centrifuge kumatsimikizira kuti amasunga mawonekedwe ake nthawi yonse yolekanitsa madzi, popanda kulekanitsidwa kapena kuwonongeka.

Kutentha kwa madzi a centrifuge kumatha kukhudza magwiridwe antchito a kulekanitsa, chifukwa kumakhudza mphamvu ya kinetic ndi momwe tinthu timakhalira. Kusunga kutentha koyenera kwa madzi a centrifuge ndikofunikira kuti tipewe kusinthasintha kulikonse pakulekanitsa. Kuphatikiza apo, kugwirizana kwa mankhwala a madzi a centrifuge ndi chitsanzo chomwe chikukonzedwa ndikofunikira kuti tipewe kusokonezeka kulikonse kapena kusokoneza kulekanitsa.

Kugwiritsa Ntchito Madzi a Centrifuge m'mafakitale Osiyanasiyana

Kugwiritsa ntchito madzi a centrifuge powonjezera mphamvu yolekanitsa madzi kumagwiritsidwa ntchito m'mafakitale osiyanasiyana komanso m'magawo ofufuza. Mu kafukufuku wa zamoyo ndi zamankhwala, madzi a centrifuge amagwiritsidwa ntchito polekanitsa magawo a subcellular, kuyeretsa mapuloteni, komanso kulekanitsa nucleic acids kuchokera ku zosakaniza zovuta. Kusankha madzi a centrifuge mu ntchito izi kumapangidwa kuti kugwirizane ndi zofunikira za chitsanzocho komanso kuyera komwe kumafunidwa kwa zigawo zolekanitsidwa.

Mu mafakitale opanga mankhwala ndi mankhwala a biopharmaceutical, zakumwa zozungulira zimagwira ntchito yofunika kwambiri pakuyeretsa ndi kulekanitsa mankhwala, katemera, ndi zinthu zamoyo. Kugwiritsa ntchito njira zozungulira za density gradient media pozungulira zipolopolo kumathandiza kuti tinthu tating'onoting'ono ndi mamolekyu enaake atuluke, zomwe zimapangitsa kuti pakhale mankhwala abwino kwambiri.

Magawo osamalira zachilengedwe ndi madzi otayira amagwiritsa ntchito madzi oyeretsera kuti alekanitse ndi kuyeretsa zinthu zodetsa kuchokera ku madzi ndi zinyalala zamafakitale. Pogwiritsa ntchito madzi oyenera oyeretsera, mafakitalewa amatha kuchotsa bwino zinthu zolimba zomwe zapachikidwa, zinthu zachilengedwe, ndi zina zodetsa kuchokera mumtsinje wamadzi, ndikuwonetsetsa kuti malamulo okhudza chilengedwe akutsatira.

Mu makampani opanga zakudya ndi zakumwa, zakumwa zozungulira zimagwiritsidwa ntchito polekanitsa zinthu zolimba ndi zamadzimadzi popanga madzi amadzimadzi, mafuta, ndi mkaka. Pogwiritsa ntchito zakumwa zozungulira zokhala ndi zinthu zopangidwa mwaluso, monga kukhuthala ndi kuchulukana, opanga amatha kupeza kulekanitsa bwino zinthuzo pamene akusunga ubwino ndi thanzi la zinthu zomaliza.

Chidule

Pomaliza, kugwiritsa ntchito madzi ozungulira ndi chinthu chofunikira kwambiri pakuwonjezera mphamvu yolekanitsa madzi mu njira zozungulira. Kusankha madzi ozungulira ndi chinthu, kutengera momwe amagwirira ntchito komanso momwe amagwirizanirana ndi chitsanzocho, kungakhudze kwambiri ubwino ndi liwiro la kulekanitsa. Pomvetsetsa mfundo zomwe zili kumbuyo kwa centrifugation ndi ntchito ya madzi ozungulira ndi chinthu, mafakitale ndi malo ofufuzira amatha kukonza njira zawo zolekanitsa madzi kuti apeze zotsatira zabwino kwambiri pa ntchito zosiyanasiyana. Pamene ukadaulo ukupita patsogolo komanso kufunikira kwa njira zolekanitsa molondola kukukula, ntchito ya madzi ozungulira ndi chinthu pakugwiritsa ntchito bwino madzi ipitiliza kukhala chinthu chofunikira kwambiri pakupanga zinthu zatsopano komanso chitukuko m'munda wa centrifugation.