Isetshenziswa kanjani i-centrifuge yeshubhu ekuhlukaniseni igazi lesilwane?

Kwezokwelapha kwezilwane kanye nocwaningo lwezokwelapha, ukuhlukanisa igazi lezilwane ngempumelelo libe yi-plasma, amangqamuzana abomvu egazi, kanye nama-platelet kubalulekile ekuxilongweni nasekuthuthukisweni kokwelashwa. Ama-centrifuge e-tubular—anejubane eliphezulu kakhulu kanye nokunemba kwawo—ayithuluzi elisetshenziswa kakhulu kuma-lab emhlabeni jikelele. Kodwa asebenza kanjani, futhi yini ewenza afaneleke ukuphatha amasampula egazi? Ake sihlaziye isayensi.

Isimiso sokuhlukanisa se-centrifuge yeshubhu

Ama-centrifuge e-tubular ahlukanisa izingxenye zegazi ngokusebenzisa umehluko wobuningi. Lapho isigubhu sijikeleza ngesivinini esikhulu, sikhiqiza amandla e-centrifugal, okubangela ukuthi izakhi ezixineneyo (njengamaseli abomvu egazi) zithuthele phansi kwepayipi, kuyilapho izingxenye ezikhanyayo (njenge-plasma) zihlala eduze kwaphezulu.

Igazi lezilwane liqukethe i-plasma namaseli egazi—kufaka phakathi amaseli abomvu egazi, amaseli amhlophe egazi, nama-platelet. Njengoba lezi zingxenye zinobukhulu obuhlukahlukene, i-centrifugation ivumela ukuhlukaniswa okuphumelelayo kwezicelo zokuxilonga nokucwaninga.

Izici ze-Tubular centrifuge

Ukusebenza kahle kokuhlukanisa kwe-Tubular centrifuge: ijubane le-tubular centrifuge livame ukufika ezinkulungwaneni zokujikeleza ngomzuzu, lingakhiqiza amandla aqinile e-centrifugal, ngesikhathi esifushane ukuze kufezwe ukuhlukaniswa okuphumelelayo kwezingxenye zegazi.

- Umphumela omuhle wokuhlukaniswa kwe-tubular centrifuge: amangqamuzana abomvu egazi, amangqamuzana amhlophe egazi, ama-platelet kanye ne-plasma egazini kungahlukaniswa ngokucacile ukuze kuhlangatshezwane nezidingo ezahlukene zokuhlolwa kwezokwelapha kanye nokwelashwa.

- I-Tubular centrifuge kulula ukuyisebenzisa: imishini inezinga eliphezulu lokuzenzakalela, vele ufake isampula yegazi epayipini le-centrifuge, usethe amapharamitha, bese uqala i-centrifuge.

Ngokungafani nokufakwa kwenhlabathi, ama-centrifuge eshubhu athola ukuhlukana okusheshayo ngenxa yama-RPM aphezulu).

Isimo sokusetshenziswa kwe-Tubular centrifuge

- Kusetshenziswa ekuhlolweni kwezokwelapha: ekuhlolweni kwegazi okuvamile, ekuhlaziyweni kwegazi ngamakhemikhali, njll. Ngokuhlukanisa izingxenye zegazi, kungatholakala izinkomba ezahlukene zamakhemikhali e-plasma, futhi amaseli egazi angabonwa futhi abalwe ukuze kunikezwe isisekelo sokuxilongwa kwesifo.

- Ukusetshenziswa ekulungiseleleni imikhiqizo yegazi: Ekulungiseleleni imikhiqizo yegazi efana namaprotheni e-plasma kanye nezinto ezihlanganisa ukujiya kwegazi, ama-centrifuge e-tubular angahlukanisa i-plasma egazini lonke ukuze ahlinzeke ngezinto zokusetshenziswa zezinqubo ezilandelayo zokuhlanza nokulungiselela.

- Ukusetshenziswa ekuhlukanisweni kwamaseli e-stem: Emkhakheni wocwaningo kanye nokwelashwa kwamaseli e-stem, amaseli e-hematopoietic stem angahlukaniswa negazi ngama-tube centrifuges ukuze ahlinzeke ngemithombo yamaseli yokufakelwa kanye nokwelashwa kwamaseli e-stem.

Izinyathelo zokuphepha zokuhlukaniswa kwegazi nge-tubular centrifuge

- Ukwelashwa kwesampula ngaphambi kokuhlukaniswa: Amasampula egazi lezilwane kufanele ahlukaniswe ngesikhathi ngemva kokuqoqwa ukuze kugwenywe izinguquko ekwakhekeni kwegazi okubangelwa ukubekwa isikhathi eside. Ngesikhathi esifanayo, ukwelashwa kwesampula nge-anticoagulant kufanele kwenziwe ngokuhambisana nezinqubo zokusebenza ukuvimbela ukujiya kwegazi ukuthi kungathinti umphumela wokuhlukaniswa.

- Ukulungiswa kwemishini ye-Tubular centrifuge: Ukulungiswa njalo nokugcinwa kwe-tubular centrifuge, okuhlanganisa ukuhlanza igumbi le-centrifugal, ukufaka esikhundleni izingxenye ezigugile, isivinini sokulinganisa, njll., ukuqinisekisa ukusebenza okuzinzile kwemishini kanye nomphumela wokuhlukanisa onokwethenjelwa.

- Ukuphepha kwegazi: Naka ukuvikelwa kokuphepha kwegazi ngesikhathi sokuhlukaniswa kwegazi ukuze uvimbele ukungcoliswa kwezinto eziphilayo okubangelwa ukuvuza kwesampula yegazi. Ngemva kokusebenzisa, amashubhu e-centrifugal nezinto ezisetshenziswayo ezihlobene nawo kufanele zilahlwe ngendlela efanele ngokuhambisana nemithethonqubo yokulahla imfucuza yezokwelapha.