kungani i-plasma yegazi ingahlukani ku-centrifuge

Ukuqonda Ukwakheka Kwe-Plasma Yegazi

Indima Yokuhlukaniswa Kwegazi Ekuhlukanisweni Kweplasma Yegazi

Amandla Ahilelekile Ekuqiniseni I-Centrifugation kanye Nokuzinza Kwe-Plasma

Izici Ezithinta Ukuhlukaniswa Kwe-Plasma Ku-Centrifuge

Intuthuko Ekusetshenzisweni Kwe-Centrifugation Ekuhlukanisweni Kwe-Plasma Okuthuthukisiwe

Isingeniso:

I-plasma yegazi, ingxenye ewuketshezi yegazi, idlala indima ebalulekile ekuthuthweni kwezinto ezahlukahlukene emzimbeni wonke. Lapho igazi lifakwa ku-centrifugation, lihlukana libe yizingqimba ezihlukene ngenxa yokwehluka kobuningi. Kodwa-ke, into eyodwa ethakazelisayo eye yadida ososayensi iminyaka eminingi ukuthi kungani i-plasma yegazi ingahlukani ibe yizingqimba ezihlukene lapho ifakwa ku-centrifuge. Kulesi sihloko, sizohlola izizathu zalokhu kuziphatha okudidayo futhi sihlolisise isayensi ngemuva kokuzinza kwe-plasma yegazi ku-centrifuge.

Ukuqonda Ukwakheka Kwe-Plasma Yegazi:

I-plasma yegazi yakhiwe ngokuyinhloko ngamanzi, ama-electrolyte, amaprotheni, ama-hormone, imikhiqizo engcolile, kanye nezakhamzimba. Isebenza njengendlela yokuthutha lezi zingxenye ziye ezingxenyeni ezahlukene zomzimba. Amaprotheni aku-plasma, njenge-albumin, ama-globulin, kanye ne-fibrinogen, anendima ebalulekile ekuphenduleni komzimba ekulweni namasosha omzimba, ekuqineni kwegazi, nasekugcineni ibhalansi ye-osmotic. Lezi zingxenye ezahlukahlukene zinegalelo ezimpahleni ezihlukile ze-plasma kanye nokuziphatha kwayo ngesikhathi sokusebenza kwe-centrifugation.

Indima Yokuhlukaniswa Kwegazi Ekuhlukanisweni Kwe-Plasma:

I-Centrifugation iyindlela ebalulekile esetshenziswa kabanzi emalabhorethri ezokwelapha nocwaningo ukuhlukanisa izinto ezinobukhulu obuhlukene. Endabeni yegazi, i-centrifugation ivumela ukuhlukaniswa kwe-plasma ezingxenyeni zamaseli okuhlanganisa amangqamuzana egazi abomvu namhlophe. Ngokuvamile, lapho igazi lifakwa i-centrifuge, lihlukana libe yizingqimba ezintathu ezihlukene: amangqamuzana egazi abomvu phansi, ungqimba oluncane lwamangqamuzana egazi amhlophe nama-platelet ngaphezu kwawo, kanye ne-plasma phezulu. Kodwa-ke, lokhu kuhlukaniswa okucacile akwenzeki nge-plasma, okuphakamisa imibuzo ethakazelisayo ngamandla ahilelekile kule nqubo.

Amandla Ahilelekile Ekuqiniseni I-Centrifugation kanye Nokuzinza Kwe-Plasma:

I-Centrifugation isebenzisa isimiso somzimba samandla e-centrifugal ukuhlukanisa izinto ngokusekelwe ekubuneni kwazo. Ku-centrifuge, lapho i-rotor ijikeleza ngesivinini esikhulu, inamandla asunduzela izinhlayiya ezixineneyo phansi, yakha izendlalelo ezihlukile. Nokho, i-plasma yegazi ayakhi izendlalelo ezihlukene ngenxa yokwakheka kwayo okuyinkimbinkimbi kanye nokuqina.

Ukuqina kwe-plasma ngesikhathi sokusebenza kwe-centrifugation kungabangelwa yizici eziningi. Ngokungafani namaseli egazi abomvu namhlophe, anobuningi obuhlukile, amaprotheni nezinye izingxenye ku-plasma zinezinhlobo eziningi zobukhulu. Ngaphezu kwalokho, i-plasma iqukethe ama-electrolyte asiza ukugcina ibhalansi ye-osmotic edingekayo ukuze kube nokuqina. Ukuba khona kwalokhu kuqina okuguqukayo kanye nokwakheka okulinganiselayo kwe-plasma kwenza ikwazi ukumelana nokuhlukana ngisho nangaphansi kwamandla aphezulu e-centrifugal.

Izici Ezithinta Ukuhlukaniswa Kwe-Plasma Ku-Centrifuge:

Nakuba i-plasma yegazi ihlala izinzile ngesikhathi sokusebenza kwe-centrifugation, izici ezithile zingaphazamisa lokhu kuzinza futhi ziholele ekuhlukanisweni kwezingqimba. Esinye sezici ezinjalo isikhathi - ukusebenzela i-centrifugation isikhathi eside kungagcina kuholele ekuhlukanisweni kwe-plasma, yize kungesivinini esiphansi uma kuqhathaniswa nezinye izingxenye zegazi. Ijubane le-centrifuge lingenye inguquko ebalulekile. Uma amandla e-centrifugal asetshenziswayo enganele, i-plasma ingase ingahlukani ngokucacile.

Esinye isici esingathinta ukuhlukaniswa kwe-plasma ukuba khona kwama-anticoagulant noma izithasiselo kusampula yegazi. Lezi zinto zingaphazamisa ukuzinza kwezingxenye ze-plasma, okuholela ekwakhekeni kwengqimba. Ngaphezu kwalokho, ubukhulu nesimo sethubhu lokuqoqwa kwegazi kungathinta ukuzinza kwe-plasma. Amathubhu angaxubeki ngokwanele ngesikhathi nangemva kokucutshungulwa kwe-centrifugation angavimbela ukuhlukaniswa kwe-plasma.

Intuthuko ku-Centrifugation Yokuhlukaniswa Kwe-Plasma Okuthuthukisiwe:

Ukuze banqobe imikhawulo ekuhlukanisweni kwe-plasma, abacwaningi bebehlola amasu amasha ekuhlukaniseni i-centrifugation. Ukuthuthukiswa komklamo we-rotor, ukulawula isivinini, kanye nokuqapha okunembile ngesikhathi sokuhlanganisa i-centrifugation kuveze ukuhlukaniswa kwe-plasma okuthuthukisiwe. Ngaphezu kwalokho, ukuthuthukiswa kwamashubhu e-centrifuge akhethekile kanye nezengezo kuthuthukise ukuzinza kwe-plasma ngesikhathi sokuhlanganisa i-centrifugation, okuholele ekuhlukanisweni okucacile.

Isiphetho:

I-plasma yegazi, naphezu kokwakheka kwayo okuyinkimbinkimbi, ayihambisani nokuhlukaniswa kwezingqimba okuvamile okubonwayo lapho ifakwa ku-centrifuge. Ubuningi obuguquguqukayo bezinto ezisetshenziswayo, kanye nezici eziqinisayo ezifana namaprotheni nama-electrolyte, kunegalelo ekuzinzeni kwayo. Ngenkathi amasu okufaka ku-centrifuge eqhubeka nokukhula, ukuqonda isayensi ngemuva kokuziphatha kwe-plasma yegazi ku-centrifuge kunemiphumela ethakazelisayo kwezokwelapha, ucwaningo, kanye nezicelo zokuxilonga.