ingabe izimvula zingahlukaniswa nge-centrifuge

Isihloko:

Isingeniso:

I-Centrifugation iyindlela esetshenziswa kabanzi ezimbonini ezahlukene, okuhlanganisa nemithi, i-biotechnology, kanye ne-chemistry. Ihilela ukuhlukaniswa kwezingxenye engxubeni ngokusekelwe ekuhlukeni kwazo kobuningi. Umbuzo owodwa othakazelisayo ophakamayo ukuthi ingabe ama-precipitate angancibiliki angahlukaniswa ngempumelelo kusetshenziswa i-centrifuge. Kulesi sihloko, sizohlola amathuba kanye nemikhawulo ye-centrifugation yokuhlukanisa ama-precipitate angancibiliki.

1. Ukuqonda Ama-Insoluble Precipitates:

Ukuze uqonde ukuthi ama-precipitate angancibiliki angahlukaniswa yi-centrifuge, kubalulekile ukuqonda kuqala ukuthi la ma-precipitate ayini. Ama-precipitate angancibiliki ayizinhlayiya eziqinile ezakha lapho izinto ezimbili noma ngaphezulu ezincibilikayo zisabelana. Lezi zinhlayiya azincibiliki ku-solvent futhi zivame ukuhlala phansi kwesitsha. Ukuzihlukanisa nesigaba soketshezi kungaba yinselele ngaphandle kokusebenzisa amasu athile njengo-centrifugation.

2. Isimiso Sokugqamisa:

I-Centrifugation isebenzisa isimiso se-sedimentation, lapho izinhlayiya ezingxubeni zoketshezi zivame ukuhlala khona ngenxa yamandla adonsela phansi. I-centrifuge isheshisa le nqubo ngokusebenzisa amandla e-centrifugal, aqhubela izinhlayiya ezindongeni ze-centrifuge tube. Amandla asetshenziswayo ancike esivinini sokujikeleza kanye ne-radius ye-centrifuge rotor. Ngokusebenzisa le ndlela, izinto zingahlukaniswa ngokusekelwe ekuhlukeni kwazo kobuningi. Kodwa-ke, ama-precipitates anganyibiliki anezici ezihlukile ezingaba yizinselele ekuhlukaneni kwawo.

3. Izinselele Zokuhlukanisa Amanzi Anganyibiliki:

Ukuhlukanisa ama-precipitate angancibiliki kusetshenziswa i-centrifugation kungabhekana nezithiyo ezimbalwa. Okokuqala, ama-precipitate avame ukuba nesivinini esikhulu sokuhlala, okwenza kube nzima ukuthi i-centrifuge isebenzise amandla anele ukulwa nokuthungwa kwawo. Ngaphezu kwalokho, ubukhulu nesimo sama-precipitate kungathinta ukusebenza kahle kwenqubo ye-centrifugation. Ama-precipitate amakhulu futhi anomumo ongavamile angase angahlukani kahle, okuholela emiphumeleni emibi. Ngaphezu kwalokho, amanye ama-precipitate angakha ama-aggregate, okwenza kube nzima kakhulu ukuhlukana kwawo nge-centrifugation.

4. Ukuthuthukisa Ukwehlukana Okusebenzayo:

Naphezu kwezinselele, kungasetshenziswa amasu amaningana ukuze kuthuthukiswe ukuhlukaniswa kwama-precipitate angancibiliki kusetshenziswa i-centrifuge. Enye indlela enjalo ukufaka ama-flocculating agents noma ama-coagulant. Lezi zinto zenza kube lula ukuhlanganiswa kwezinhlayiya ezincane zama-precipitate zibe yizinqwaba ezinkulu nezilula ukuzihlukanisa. Ngokwakha ama-aggregate amakhulu, ama-precipitate aba mancane amathuba okuphinda amiswe ngesikhathi se-centrifuge, ngaleyo ndlela kuthuthukiswe ukusebenza kahle kokuhlukaniswa.

5. Izinhlobo ze-Centrifuges zokuhlukanisa imvula:

Izinhlobo ezahlukene zama-centrifuge zingasetshenziswa ukuhlukanisa ama-precipitate angancibiliki, ikakhulukazi kuye ngokuthi ivolumu kanye nezici zesampula zingakanani. Ama-centrifuge anesivinini esikhulu avame ukusetshenziswa ezindaweni zokucwaninga lapho kucutshungulwa khona inani elincane lamasampula. Lawa ma-centrifuge angafinyelela isivinini esiphezulu sokujikeleza, okuholela emandleni aqinile e-centrifugal ukuze kuhlukaniswe kahle. Ngakolunye uhlangothi, izinqubo ezinkulu zezimboni zingasebenzisa ama-centrifuge aqhubekayo, anikeza amandla aphezulu kanye nokukhula okungcono.

Isiphetho:

Ekuphetheni, nakuba ukuhlukanisa ama-precipitate angancibiliki kungaba yinselele, i-centrifugation ingaba yindlela ewusizo yokuwahlukanisa ngaphansi kwezimo ezifanele. Izici ezifana nokuxazulula ijubane, usayizi wezinhlayiya, kanye nesimo zidlala indima ebalulekile ekunqumeni impumelelo yenqubo yokuhlukanisa. Ngokusebenzisa amasu okwenza ngcono njenge-flocculation kanye nokusebenzisa uhlobo olufanele lwe-centrifuge, ama-precipitate angancibiliki angahlukaniswa ngempumelelo, okwenza izimboni ezahlukene zikwazi ukwenza lula izinqubo zazo futhi zithuthukise ikhwalithi yomkhiqizo.