I-Basket Centrifuge ye-E. coli Harvest & Clarification: Isisombululo esisebenza kakuhle esivela kwiZonelink

I-Basket Centrifuge ye-E. coli Harvest & Clarification: Isisombululo esisebenza kakuhle esivela kwiZonelink

Kwintsimi ye-biotechnology ekhula ngokukhawuleza namhlanje, i-Escherichia coli (E. coli), njengeyona nkqubo yokubonakalisa i-prokaryotic eqhelekileyo nesetyenziswa kakhulu, idlala indima ephambili ekuvelisweni kweeproteni eziphinda zihlanganiswe, ii-vaccines, ii-enzymes, kunye nezinto ezahlukeneyo ezisebenzayo kwi-biotechnology.

Nangona kunjalo, ukubuyisela imveliso ekujoliswe kuyo ngokufanelekileyo nangobunono kwinkqubo yomhluzi ontsonkothileyo emva kokubiliswa okukhulu kusengumngeni omkhulu kwiinkqubo zokucoca ezisezantsi.

Namhlanje, siza kubonisa indlela ii-Lianning Zonelink Basket Centrifuges, ngokusebenza kwazo okugqwesileyo, eziba lukhetho olufanelekileyo lokuvuna kunye neenkqubo zokucacisa i-E. coli.

Umngeni Wokuvuna I-E. coli: Kutheni Ukhetha Ukwahlulwa Kwe-Centrifugal?

Umhluzi wokubilisa i-E. coli ngumxube ontsonkothileyo oqulethe iiseli, i-unconsumated culture medium, ii-metabolites, kunye neproteni ekujoliswe kuyo. Iindlela zemveli ezifana nokuqunjelwa kunye nokuhluzwa zihlala zijongene nemingeni efana nokusebenza okuphantsi, ukungakwazi ukuvala, kunye nokulahlekelwa ngumsebenzi xa kujongwana nemihluzi yokubilisa enkulu, enoxinano olukhulu.

Ukwahlulwa kwe-centrifugal, ingakumbi kusetyenziswa ii-basket centrifuges, kuye kwaba yindlela ekhethwayo kwimveliso yemizi-mveliso ngenxa yokusebenza kwayo rhoqo, ukusebenza kakuhle kokucubungula, ukulula kokwandisa, kunye nokuhambelana okugqwesileyo kwenkqubo.

Umgaqo-nkqubo osebenzayo weeBasket Centrifuges ekucutshungulweni kwe-E. coli

Umgaqo osebenzayo we-basket centrifuge yokucubungula i-E. coli usekelwe kwi-centrifugal sedimentation. Xa umhluzi we-E. coli fermentation ufakwa kwisitya esijikelezayo ngesantya esiphezulu, amandla amakhulu e-centrifugal abangela ukuba iiseli zebhaktheriya ezixineneyo zichitheke ngokukhawuleza kwaye ziqine eludongeni lwesitya.

Kwangaxeshanye, ulwelo olungenaxinano (supernatant) lwenza indandatho yolwelo ngaphezulu komaleko wobunzima beseli kwaye lukhutshwa ngesixhobo sokuskena. Esi sixhobo, esinesakhiwo saso esifutshane, siphumeza ngokufanelekileyo ukwahlulwahlulwa kolwelo oluqinileyo lweeseli kunye nolwelo olucacisiweyo.

Iingenelo ezine eziphambili zeLiaoning Zonelink Basket Centrifuges zokucubungula i-E. coli :

1. Ukusebenza kakuhle kokwahlulwahlulwa kunye ne-≥99% yokwahlulwahlulwa

Intsimi yamandla e-Centrifugal enamandla: Ivelisa amandla aphezulu e-centrifugal, ivumela ukutsalwa ngokukhawuleza nangokucokisekileyo kweeseli ezincinci ze-E. coli, iqinisekisa izinga lokubuyiselwa kweseli elingaphezulu kwe-99%.

I-Clarified Supernatant: Yahlula iiseli kwi-supernatant equlethe imveliso ekujoliswe kuyo, ibeka isiseko esiqinileyo samanyathelo okucoca alandelayo afana ne-chromatography kunye ne-ultrafiltration, inciphisa kakhulu umthwalo kwiinkqubo ezisezantsi.

2. Uyilo oluphantsi lokunciphisa iiproteni lukhusela umsebenzi weproteni

Kwiimveliso ezivezwa ngaphandle kweseli okanye ezivuza lula, amandla amakhulu okucheba oomatshini anokubangela ukuba iiproteni ziguquguquke okanye zingasebenzi. IiCentrifuges zeLiaoning Zonelink Basket zinoyilo oluchanekileyo lwe-hydrodynamic.

Inkqubo yokuhambisa isebenza kakuhle, inciphisa impembelelo yeziphumo zokucheba kwi-biomolecules ezinobuthathaka, ngaloo ndlela ikhusela ukusebenza kwemveliso.

3. Ukusebenza ngokuzenzekelayo okuqhubekekayo iiyure ezingama-24 ngosuku, iintsuku ezisi-7 ngosuku

Ngokungafaniyo nee-centrifuge zebhetshi zemveli, ii-centrifuge zebhasikithi zeLiaoning Zonelink zivumela ukondla nokukhupha amanzi rhoqo iiyure ezingama-24 ngosuku, iintsuku ezisixhenxe ngeveki.

Oku akuphuculi nje kuphela ukusebenza kakuhle kwemveliso kwaye kunciphisa umahluko phakathi kwebhetshi nebhetshi kodwa kukwenza ukuba zihambelane ngokugqibeleleyo nengcamango ephambili yokwenza izinto eziphilayo ngokuqhubekayo, kunceda amashishini ukuba andise umthamo wawo kwaye aphucule iindleko.

4. Ukukhula Okugqwesileyo kunye nozinzo lweNkqubo

Ukususela kwisikali sovavanyo lwelabhoratri ukuya kwimveliso enkulu yemizi-mveliso, iqonga le-centrifuge le-Liaoning Zonelink libonelela ngendlela yokwandisa umbane ngokuthe ngqo.

Ukusebenza okuzinzileyo kuqinisekisa ukuphinda-phinda kwenkqubo kunye nokuthobela, kuhlangabezana ngokupheleleyo neemfuno ezingqongqo zomthetho.

IiCentrifuge zeLiaoning Zonelink Basket zisetyenziswa kakhulu kwiinkqubo ezahlukeneyo ezinxulumene ne-E. coli, kuquka:

1. Ukuvunwa kweeSeli ukuze kuveliswe iiProtein eziHlanganisiweyo: Ukuvunwa kweeSeli ngokukhawuleza ukuze kuphazanyiswe iiSeli kamva kunye nokukhupha iiProtein ezinyibilikayo emzimbeni/ezomileyo.

2. Ukucaciswa komhluzi wokuveliswa kwegonyo: Ukususwa kweeseli ngokufanelekileyo kwimveliso yegonyo esekelwe kwi-E. coli ukuze kufunyanwe isisombululo se-antigen esicocekileyo.

3. Ukucutshungulwa komhluzi wokubilisa kwii-Enzymes zemizi-mveliso: Ukucutshungulwa komhluzi wokubilisa ngobuninzi ukuze kwahlulwe iiseli kwii-enzymes ezingaphandle kweseli.

4. Ukwahlulwa okuPhambili kwiPlasmid DNA Production: Ukwahlulwa kweeseli ngokufanelekileyo kwi-culture medium kwizigaba zokuqala zeenkqubo zokukhupha i-plasmid.

Ukujonga Phambili – Ukuqalisa Ikamva, Ukudala Ixabiso Ngokusebenzisana

Ngophuhliso olunamandla lwebhayoloji yokwenziwa kunye nonyango oluchanekileyo, amandla okusetyenziswa kwe-E. coli njenge "fektri yeeseli" aya kuqhubeka esanda, kwaye ubuchwepheshe bokwahlula nokucoca obuhambelanayo kufuneka buqhubeke.

I-Liaoning Zonelink iza kuqhubeka nokugxila ekuveliseni izinto ezintsha kunye nokwenza ngcono iteknoloji ye-basket centrifuge, izibophelele ekuboneleleni ngezisombululo zokwahlulahlula ezisebenzayo, ezizinzileyo, nezikrelekrele kwiinkampani ze-biotechnology zehlabathi, kunye nokukhuthaza ngokudibeneyo ukuqhubela phambili kweshishini le-biopharmaceutical.

I-Liaoning Zonelink – Umvelisi weCentrifuge oneminyaka engaphezu kwama-20, oxhobisa imveliso yakho ezinzileyo kunye nombono omkhulu

I-Liaoning Zonelink   yasekwa ngoJulayi 2001. Singumvelisi ochwephesha odibanisa uphando nophuhliso, imveliso, kunye nokuthengiswa kwezixhobo ze-centrifuge.

Iimveliso zethu ziquka iiTubular Centrifuges, iiBasket Centrifuges, iiDisc Centrifuges, kunye neeDecanter Centrifuges.

Ukuze ufunde okungakumbi malunga neemveliso zethu zebhasikithi ze-centrifuge zokucubungula i-E. coli kunye nezinye izicelo zokwahlulahlula i-bio, nceda undwendwele iwebhusayithi yethu (www.lnzonelink.com) okanye unxibelelane neqela lethu leengcali. (Nxibelelana nathi :admin@lyzhonglian.com )