yeyiphi isantya se-centrifuge sokwahlula igazi
Isantya seCentrifuge ukuya kwiGazi elahlukileyo
Intshayelelo kwiCentrifugation kunye noKwahlulwa kweGazi
Izinto Ezichaphazela Isantya Se-Centrifuge Sokwahlulwa Kwegazi
Izantya ezahlukeneyo zeCentrifuge kwiinxalenye ezithile zegazi
Ukuphucula isantya seCentrifuge ukuze kwahlulwe igazi ngokufanelekileyo
Isiphelo: Ukukhetha isantya esifanelekileyo seCentrifuge sokwahlulwa kwegazi
Intshayelelo kwiCentrifugation kunye noKwahlulwa kweGazi
Kwiindawo ezahlukeneyo zelebhu, i-centrifugation idlala indima ebalulekileyo ekwahlukaniseni iinxalenye ezahlukeneyo zesampuli yegazi. Ngokujikeleza isampuli ngokukhawuleza, iinxalenye ezixineneyo zinyanzelwa ezantsi kwetyhubhu, ngelixa iinxalenye ezikhaphukhaphu zisiya phezulu. Nangona kunjalo, ukukhetha isantya se-centrifuge esifanelekileyo kubalulekile ukuze kwahlulwe igazi ngokufanelekileyo. Eli nqaku liphonononga izinto ezichaphazela ukukhethwa kwesantya se-centrifuge kunye nendlela iisantya ezahlukeneyo ezinokuphucula ngayo ukwahlulwa kwezixhobo ezithile zegazi.
Izinto Ezichaphazela Isantya Se-Centrifuge Sokwahlulwa Kwegazi
Ukukhethwa kwesantya se-centrifuge kuxhomekeke kwizinto ezahlukeneyo, kuquka uhlobo lwe-centrifuge, umthamo wesampuli yegazi, kunye nezinto ezithile ezahlulwe. Uninzi lwee-centrifuge zanamhlanje zinezicwangciso zesantya eziguquguqukayo, ezivumela abaphandi ukuba balungelelanise inkqubo yokwahlula ngokweemfuno zabo.
Iisampuli zegazi ezinemithamo ephezulu zihlala zifuna amaxesha amade okucoca igazi kunye nesantya esiphezulu ukuze kube lula inkqubo yokwahlula igazi. Ukongeza, ukukhethwa kwesantya sokucoca igazi kuxhomekeke kuhlobo lwetyhubhu esetyenziswayo, njengoko iityhubhu ezahlukeneyo zineenkcukacha zoyilo olulodwa ezichaphazela inkqubo yokwahlula igazi. Kubalulekile ukulandela izikhokelo zomenzi xa usebenzisa iityhubhu ezithile ukuqinisekisa ukwahlula igazi ngokufanelekileyo.
Izantya ezahlukeneyo zeCentrifuge kwiinxalenye ezithile zegazi
1. Iiseli zegazi ezibomvu (ii-RBC):
Ukwahlula iiRBC kwigazi elipheleleyo, kusetyenziswa isantya se-centrifuge esimalunga ne-1500-2000 revolutions ngomzuzu (rpm). Esi santya sanele ukwahlula iiRBC ezantsi kwetyhubhu ngelixa kushiya iplasma okanye i-serum phezulu. Isantya esiphezulu sinokuchaphazela ukuthembeka kwezixhobo ezahluliweyo.
2. Iiplatelets:
Kwindlela yokwahlulwa kweeplatelet, isantya esiphantsi se-centrifuge esimalunga ne-1000-1500 rpm siyathandwa. Oku kuqinisekisa ukuba iiplatelet aziphazanyiswa okanye zonakaliswe ngexesha lenkqubo yokwahlulwa. Ekubeni iiplatelet zizakhiwo zeeseli ezibuthathaka, indlela ethambileyo enesantya esiphantsi iyanciphisa ukuphazamiseka kwazo.
3. Iiseli zegazi ezimhlophe (ii-WBC):
Ukwahlula ii-WBC, isantya se-centrifuge sidla ngokuba phakathi kwe-2000-3000 rpm. Ezi santya ziphezulu zivumela ukwakheka kwe-WBC pellet ephumelelayo ezantsi kwetyhubhu. Ukwahlula ii-WBC kubalulekile kwiindlela ezahlukeneyo zohlalutyo lwelabhoratri, kubandakanya ukubalwa komahluko kunye ne-immunophenotyping.
4. Iplasma:
Ukuze ufumane iplasma, isantya se-centrifuge kufuneka sibekwe ngaphakathi koluhlu lwe-1500-2000 rpm. Ngesi santya, isampuli yahlulwe ibe ziingqimba ezahlukeneyo, kunye neplasma phezulu, ii-WBC kunye neeplatelets embindini, kunye nee-RBC ezantsi. Ngokusebenzisa imibhobho ngononophelo, iplasma inokudluliselwa ngokufanelekileyo nangokungangcoliswanga ukuze ivavanywe ngakumbi.
Ukuphucula isantya seCentrifuge ukuze kwahlulwe igazi ngokufanelekileyo
Ukuze kufumaneke iziphumo ezilungileyo zokwahlulwa kwegazi, kubalulekile ukuphucula isantya se-centrifuge ngokusekelwe kwiziphumo ezifunekayo. Kufuneka kuqwalaselwe izinto ezibalulekileyo:
1. Ukusebenza kakuhle kwexesha kunye neendleko:
Nangona isantya esiphezulu se-centrifuge sinokukhokelela ekuqhekekeni ngokukhawuleza, kubalulekile ukulinganisela ukusebenza kakuhle kwexesha kunye neendleko ezinxulumene noko. Amaxesha amade e-centrifugation ngesantya esiphantsi anokuba neendleko ezingcono, ingakumbi xa kujongwana nomthamo omkhulu weesampuli.
2. Umzekelo woBulungisa:
Isantya se-centrifuge kufuneka sikhethwe ngononophelo ukuqinisekisa ukuthembeka kwezakhi zegazi ezahlulweyo. Isantya esiphezulu sinokubangela i-hemolysis, siphazamise iiseli ezibomvu zegazi kwaye sitshintshe iziphumo zovavanyo. Ke ngoko, kubalulekile ukufumana isantya esifanelekileyo esahlula ngokufanelekileyo izakhi ezifunekayo ngaphandle kokubeka umgangatho wazo esichengeni.
3. Ukucaciswa kovavanyo:
Uvavanyo olwahlukeneyo lunokufuna isantya esiguquguqukayo se-centrifuge ukuze kwahlulwe iinxalenye ezithile zegazi ngokuchanekileyo. Ukuqonda iimfuno zovavanyo kunye nokulandela imigaqo-nkqubo esele imiselwe kubalulekile ekufezekiseni iziphumo ezifunekayo.
4. Izikhokelo zoMvelisi:
Ukuze kuphuculwe ukusebenza kakuhle kokwahlulwa kwegazi, kuyacetyiswa ukuba ujonge izikhokelo zomenzi we-centrifuge. Abavelisi badla ngokubonelela ngolwazi malunga nesantya esicetyiswayo se-centrifuge kwiintlobo ezahlukeneyo zeetyhubhu zesampulu, ukuqinisekisa iziphumo ezithembekileyo neziphindaphindwayo.
Isiphelo: Ukukhetha isantya esifanelekileyo seCentrifuge sokwahlulwa kwegazi
Kwicandelo lokuxilongwa kwezonyango nophando, ukuqonda isantya esifanelekileyo se-centrifuge sokwahlulwa kwegazi kubaluleke kakhulu. Ngokuqwalasela izinto ezifana nomthamo wesampulu, izinto ezifunekayo, kunye neemfuno zovavanyo, umntu unokuphucula isantya se-centrifuge aze afikelele ekuhlukanisweni okusebenzayo. Ukulinganisela ixesha kunye nokusebenza kakuhle kweendleko ngelixa egcina ukuthembeka kwesampulu kuqinisekisa iziphumo ezithembekileyo ngelixa enciphisa inkunkuma. Ngokulandela izikhokelo zomenzi kunye neenkqubo ezimiselweyo, abaphandi banokukhetha isantya se-centrifuge esifanelekileyo kwiimfuno zabo ezithile zokwahlulwa kwegazi, okuphucula ukuchaneka kunye nokuthembeka kovavanyo lwabo.
.