e hlalosang kamoo centrifuge e arolang motsoako kateng

Selelekela sa Centrifugation: Kamoo Centrifuge e Arohanyang Metswako Kateng

Ho Centrifugation ke mokhoa o sebelisoang haholo oa laboratori o sebelisang melao-motheo ea ho sedimentation le matla a centrifugal ho arola metsoako ho latela bongata ba eona. Ts'ebetso ena e bohlokoa mafapheng a 'maloa a saense le a bongaka moo ho hlokahalang karohano ea likarolo tse fapaneng ka har'a sampole bakeng sa tlhahlobo, tlhoekiso, kapa ho itšehla thajana. Sehloohong sena, re tla buisana ka melao-motheo ea motheo ea ho Centrifugation le ho hlahloba lits'ebetso le mefuta e fapaneng ea li-centrifuge tse sebelisoang liindastering tse fapaneng.

1. Melao-motheo ea ho Centrifugation

Pele re hlahloba mathata a ho centrifugation, ho bohlokoa ho utloisisa melao-motheo e ka morao ho mokhoa ona. Molao-motheo o ka sehloohong o amehang mona ke ts'ebeliso ea matla a centrifugal ho arola likarolo tsa motsoako. Ha sampole e beoa ka har'a centrifuge 'me e sothoa ka lebelo le phahameng, likaroloana tse teteaneng kapa limolek'hule li ea tlase, ha likaroloana tse se nang teteane li atisa ho lula haufi le holimo. Karohano ena e etsahala ka lebaka la ho senngoa ha likaroloana tse teteaneng, tse potlakisang matla a centrifugal.

2. Mefuta e fapaneng ea Li-Centrifuge

Ho na le mefuta e fapaneng ea li-centrifuge tse fumanehang, e 'ngoe le e 'ngoe e etselitsoe lits'ebetso tse itseng. E 'ngoe ea mefuta e sebelisoang haholo ke benchtop centrifuge, e nang le mekhoa e mengata 'me e sebelisoa lilaboratoring bakeng sa mesebetsi ea kamehla ea karohano. Mofuta o mong ke ultracentrifuge, e khonang ho fihlella lebelo le phahameng haholo le ho hlahisa matla a matla a centrifugal, e leng se etsang hore e be e loketseng mesebetsi e hlokang likaroloana tse arohaneng tse nang le boima bo tlase haholo.

Ka lehlakoreng le leng, di-centrifuge tse sehatsetsing di na le ditsamaiso tsa ho phodisa tse dumellang karohano ya disampole tse amehang mochesong ntle le ho beha botšepehi ba tsona kotsing. Di-centrifuge tsena di sebediswa haholo ditshebedisong tse kang baeloji ya dimolek'hule, moo ho boloka mocheso o nepahetseng ho leng bohlokwa.

3. Likopo tsa Bongaka

Lefapheng la tlhahlobo ea bongaka, ho thiba mali ho phetha karolo ea bohlokoa tlhahlobong le karohanong ea lisampole tsa mali. Ka ho potoloha lisampole tsa mali ka har'a centrifuge, litsebi tsa tlhokomelo ea bophelo li ka arola likarolo tse fapaneng, tse kang lisele tse khubelu tsa mali, lisele tse tšoeu tsa mali le plasma. Karohano ena e nolofalletsa ho lemoha le ho hlahloba mafu le maemo a fapaneng, hammoho le ho bokella likarolo tse itseng tsa mali bakeng sa merero ea phekolo.

4. Likopo tsa Liindasteri

Ho thiba metsi ka sekontiri ho boetse ho sebelisoa haholo lits'ebetsong tse fapaneng tsa indasteri. Mohlala, indastering ea lijo le lino, li-centrifuge li sebelisoa ho arola metsi ho lintho tse tiileng le ho hlakisa lero, veine le lino tse ling. Ho feta moo, li-centrifuge li bapala karolo ea bohlokoa lifemeng tsa kalafo ea metsi a litšila, moo li thusang ho arola lintho tse tiileng ho metsi, li nolofalletsa ho lahla kapa ho sebelisa hape metsi a hloekisitsoeng ka mokhoa o sireletsehileng.

5. Patlisiso le Biotechnology

Lilaboratoring tsa lipatlisiso le litsing tsa theknoloji ea baeloji, centrifugation e sebelisoa haholo lits'ebetsong tse fapaneng. Hangata e sebelisoa ho hloekisa liprotheine, ho arola li-organelle tsa sele, ho arola li-nucleic acid, le ho bokella lisele ho tsoa mecheng ea temo ea sele. Mekhoa ena ea bohlokoa bakeng sa ho etsa liteko, ho ithuta likarolo tse itseng tsa sele le ho nts'etsapele litharollo tsa kalafo.

Qetello

Ho centrifugation ke mokhoa oa bohlokoa o lumellang karohano ea metsoako lits'ebetsong tse 'maloa tsa saense, tsa bongaka le tsa indasteri. Ka ho sebelisa matla a matla a centrifugal, bafuputsi le litsebi mafapheng a fapaneng ba ka arola le ho sekaseka likarolo tse fapaneng ka har'a sampole ka nepo. Ho tenyetseha le mefuta e mengata ea ts'ebeliso ea centrifugation li etsa hore e be sesebelisoa sa bohlokoa lilaboratoring lefatšeng ka bophara, ho thusa mesebetsing e tlohang lits'ebetsong tse tloaelehileng tsa tlhahlobo ho isa lipatlisisong tse ncha.