centrifuge e arola thepa ho latela bongata ba tsona

Sentrifuge e Arohanya Thepa ho latela Botebo ba Tsona

Selelekela

Lefapheng la mahlale le lipatlisiso, mekhoa le lisebelisoa tse fapaneng li ntlafalitsoe ho arola le ho sekaseka lintho tse fapaneng. Har'a mekhoa ena, tšebeliso ea centrifuge e hlahella e le mokhoa o atlehang oa ho arola thepa ho latela bongata ba tsona. Centrifuge ke sesebelisoa sa laboratori se sebelisoang haholo se sebelisang matla a centrifugal ho arola metsoako e fapaneng ka katleho. Sehloohong sena, re tla hlahloba melao-motheo ea centrifugation, ts'ebeliso ea eona masimong a fapaneng, mefuta e fapaneng ea centrifuges, bohlokoa ba mecha e nepahetseng ea gradient ea density, 'me qetellong, mehato e meng ea ts'ireletso eo u lokelang ho e nahana ha u ntse u sebetsana le sesebelisoa sena se matla.

Ho Utloisisa Centrifugation: Lintho tsa Motheo

Ho centrifugation ke mokhoa oa karohano o sebelisang molao-motheo oa ho sedimentation. Ha motsoako o kenngoa tlas'a matla a centrifugal, likarolo tse teteaneng li atisa ho sediment kapele ho feta likarolo tse bobebe. Karohano ena e nolofalloa ke motsamao oa potoloho o hlahisoang ka har'a centrifuge, e leng se fellang ka matla a matla a centrifugal a sebetsang lisampoleng.

Likopo mafapheng a fapaneng

1. Lipatlisiso le Tlhahlobo ea Bongaka: Ho sebelisa centrifugation ho bapala karolo ea bohlokoa lipatlisisong tse fapaneng tsa bongaka le lits'ebetsong tsa tlhahlobo. Ho sebelisoa haholo ho aroleng le ho hloekiseng DNA, RNA, liprotheine le likarolo tse ling tsa sele. Ho feta moo, ho bohlokoa haholo ho hlahlobeng mafu ka ho arola likarolo tsa mali, tse kang plasma, lisele tse khubelu tsa mali le lisele tse tšoeu tsa mali.

2. Indasteri ea Meriana: Indastering ea meriana, ho chencha metsoako ea meriana ho sebelisoa haholo-holo ts'ebetsong ea tlhahiso ho arola le ho hloekisa metsoako e sebetsang ea meriana (API). E thusa ho arola lintho tse tiileng ho tse metsi, ho netefatsa hore litšila lia tlosoa le ho fihlela boleng bo holimo ba sehlahisoa.

3. Tlhahlobo ea Tikoloho: Ho kenya centrifugation le hona ho bohlokoa haholo tlhahlobong ea tikoloho. Ho nolofalletsa karohano ea lintho tse tiileng tse emisitsoeng ho tsoa lisampoleng tsa metsi, ho thusa ho fumana maemo a litšila le ho netefatsa liteko tse nepahetseng tsa tikoloho.

4. Indasteri ea Lijo le Lino: Indasteri ea lijo le lino e sebelisa haholo mokhoa oa ho centrifugation ho arola likarolo tse fapaneng nakong ea ts'ebetso ea tlhahiso. E sebelisetsoa ho arola mafura, liprotheine le likaroloana tse ling tse sa batleheng, e leng se fellang ka boleng bo ntlafetseng ba sehlahisoa le nako e telele ea ho se boloka.

5. Indasteri ea Petrochemical: Ho sebelisa centrifugation ho phetha karolo ea bohlokoa indastering ea peterole ka ho nolofatsa karohano ea oli e tala ka likaroloana tse fapaneng, tse kang peterole, diesel, mafura le oli e futhumatsang. Ts'ebetso ena ea karohano e bohlokoa bakeng sa ho hloekisa le ho netefatsa boleng ba lihlahisoa tsa ho qetela.

Mefuta e fapaneng ea li-Centrifuge

1. Li-Ultracentrifuge: Tsena ke li-centrifuge tse lebelo le phahameng tse khonang ho fihlella lebelo le phahameng haholo la ho potoloha, hangata li pakeng tsa li-revolutions tse 60,000 ho isa ho tse 150,000 ka motsotso (rpm). Li sebelisetsoa ho arola le ho hlahloba litšobotsi tsa limolek'hule tse kang DNA, liprotheine le likaroloana tse nyane.

2. Di-Centrifuge tse Sehatsetsing: Di-centrifuge tse sehatsetsing di na le dikarolo tsa taolo ya mocheso, tse dumellang ho sebetswa ha disampole tse amehang mochesong. Ho seha mocheso ka serame ho thusa ho boloka botsitso ba disampole nakong ya ditshebetso tsa karohano.

3. Li-Centrifuge tsa Benchtop: Li-centrifuge tsena li nyane, lia nkeha, 'me li fumanoa hangata lilaboratoring tsa lipatlisiso. Li fana ka bokhoni bo tlase ba lebelo empa lia tenyetseha ebile li loketse mefuta e mengata ea likarohano tsa kamehla, ho kenyeletsoa le ho lokisa sampole le tlhahlobo ea bongaka.

4. Li-Microcentrifuge: Li etselitsoe ka ho khetheha li-volume tse nyane tsa lisampole, li-microcentrifuge li tsebahala ka bokhoni ba tsona ba lebelo le leholo le sebaka se senyenyane. Hangata li sebelisoa lilaboratoring tsa baeloji ea limolek'hule le biochemistry bakeng sa ho lokisa sampole ka potlako le ka katleho.

5. Li-Centrifuge tsa ho Lokisetsa: Li-centrifuge tsena li sebelisoa ha bongata bo boholo ba sampole bo hloka ho sebetsoa le ho aroloa. Hangata li fumanoa libakeng tsa indasteri 'me li fana ka matla a phahameng a centrifuge le bokhoni bo boholo.

Bohlokoa ba Mecha ea Litaba ea Gradient e Tenyetsehang

Mecha ea phatlalatso ea gradient e teteaneng e bapala karolo ea bohlokoa ts'ebetsong ea karohano ka har'a centrifuge. Methati ea gradient e teteaneng e lokisoa ka ho kenya litharollo tsa methati e fapaneng ka har'a tube ea centrifuge. Ha sampole e kenngoa centrifuge, likaroloana li falla ka har'a gradient ea methati tlas'a tšusumetso ea matla a centrifugal. Ts'ebetso ena e thusa haholo ha ho aroloa likaroloana tse nang le methati e tšoanang. Methati ea gradient e teteaneng e lumella karohano e nepahetseng le e sebetsang ea lisampole ho latela methati ea tsona e ikhethang.

Mekhoa ea ho Hlokomela Tšireletso ha U Sebetsana le Li-Centrifuge

1. Tsamaiso e Nepahetseng ea Lisebelisoa: Netefatsa koetliso e nepahetseng ea mokhoa oa ho sebetsana le lisebelisoa tsa centrifuge. Latela litataiso tsa moetsi bakeng sa ho kenya lisampole, ho leka-lekanya liphaephe tsa rotor, le ho beha lebelo le mocheso o nepahetseng.

2. Ho Tsamaellana le ho Hlahloba Rotor: Pele ho tshebediso ka nngwe, hlahloba rotor mme o netefatse hore e tsamaellana le ditlhoko tsa lebelo le bokgoni ba disampole tse ntseng di sebetswa. Tshenyo efe kapa efe e bonahalang kapa matshwao a ho tsofala a lokela ho hlahlojwa ke setsebi se tshwanelehang pele se sebediswa.

3. Tšebeliso e Nepahetseng ea Lisebelisoa tsa Polokeho: Kamehla apara lisebelisoa tse loketseng tsa tšireletso tse kang liatlana, lijase tsa laboratori le likhalase tsa tšireletso. Ho feta moo, ela hloko liaparo tse hlephileng le mabenyane a ka ’nang a tšoasoa ke likarolo tse tsamaeang.

4. Kabo e Lekaneng ea Moroalo: Ho bohlokoa ho netefatsa hore mojaro o ka har'a rotor ea centrifuge o leka-lekane. Ho jara ka mokhoa o sa lokelang ho ka lebisa ho hloleheng ha lisebelisoa, karohano e sa lekanang le likotsi tse ka bang teng. Sheba bukana ea tataiso ea centrifuge bakeng sa litaelo tse tobileng mabapi le kabo e nepahetseng ea mojaro.

5. Ho Hloekisa le ho Hlokomela: Hlahloba le ho hlwekisa dikarolo tsa centrifuge kgafetsa, ho kenyeletswa le dibakete tsa rotor, di-gasket le kamore, ho qoba tšilafalo le ho netefatsa tshebetso e ntle. Latela ditaelo tsa moetsi bakeng sa tlhokomelo le tshebeletso ya kamehla.

Qetello

Ho centrifugation ke mokhoa oa bohlokoa mafapheng a mangata a saense, o nolofalletsang karohano ea thepa ho latela bongata ba eona. Ts'ebeliso ea eona e akaretsa liindasteri tse fapaneng, e nolofalletsa lipatlisiso, tlhahlobo, tlhahiso le lits'ebetso tsa taolo ea boleng. Ka mefuta e fapaneng ea li-centrifuge tse fumanehang, bo-rasaense le bafuputsi ba ka fetola mekhoa ea bona ea karohano ho latela litlhoko tse itseng. Ka ho utloisisa melao-motheo le ho latela mehato ea ts'ireletso, bafuputsi ba ka sebelisa matla a centrifugation ho ntšetsa pele tsebo ea saense le ho kenya letsoho masimong a mangata.