iri kushandisa nzira yekuparadzanisa centrifuge

Kuisa Centrifuging: Nzira Yakakosha Yekuparadzanisa Mumaindasitiri Akasiyana-siyana

Nhanganyaya

Kuparadzanisa zvinhu, nzira yekuparadzanisa inoshandiswa zvakanyanya mumaindasitiri akadai semishonga, biotechnology, uye manejimendi emarara, yakachinja nzira yatinoparadzanisa zvinhu zvichienderana nehuwandu hwazvo. Muchinyorwa chino, tichaongorora mashandisirwo, misimboti, uye mabhenefiti ekubatanidza zvinhu senzira yekuparadzanisa, pamwe chete nemhedzisiro yayo pazvikamu zvakasiyana. Tichaongorora mhando dzakasiyana dzema centrifuge aripo uye toongorora mashandiro adzo. Pamusoro pezvo, tichakurukura mamwe matekiniki epamusoro uye mafambiro ari kubuda mu centrifugation. Ngatitangei rwendo urwu rwekunzwisisa munyika ye centrifugation!

Kunzwisisa Centrifugation neMitemo Yayo Yekutanga

Kuparadzanisa zvinhu uchishandisa nzira ye "centrifugation" (centrifugation) inzira yekuparadzanisa zvinhu uchishandisa simba re "centrifugal" (centrifugation) kuti zviparadzanise zvinhu zvichienderana nehuremu hwazvo kana kuti kuwanda kwazvo. Maitiro aya anosanganisira kutenderedza musanganiswa nekumhanya kwakanyanya, zvichigadzira munda we "centrifugation" unokurudzira kugadzikana kwezvinhu zvinorema zvichienda pasi. Nekushandisa musimboti we "sedimentation", kuparadzanisa zvinhu kunoita kuti zvisanyanya kurema kana kuti zvinhu zvine huwandu hwakasiyana.

Mashandisirwo eCentrifuging muMishonga neBiotechnology

Kugadzira mishonga ye "centrifuge" kunoita basa guru mukushandiswa kwakasiyana-siyana mumaindasitiri emishonga ne biotechnology. Mukutsvaga mishonga, "centrifuge" dzinoshandiswa kuparadzanisa nekuparadzanisa zvinhu zvakasanganiswa kubva mumisanganiswa yakaoma. Pamusoro pezvo, dzinoshandiswa mukugadzira zvinhu zviri pasi, zvichiita kuti masero, mapuroteni, uye nucleic acids zviparadzaniswe. "Centrifuge" dzinoshandiswawo mukugadzira majekiseni, zvichibvumira kubviswa kwetsvina uye kuona kuchena kwakanyanya muchigadzirwa chekupedzisira.

Kudzora marara neSainzi yezvakatipoteredza

Basa rekutarisira marara rinobatsirwa zvikuru nekushandisa centrifugation, sezvo ichipa mhinduro dzinoshanda dzekuparadzanisa nzvimbo dzekuchenesa marara. Centrifuge dzinobatsira mukubvisa mvura mumarara, kuparadzanisa zvinhu zvine mvura nezvine simba, nokudaro dzichideredza huwandu hwemarara. Maitiro aya haangoderedzi chete kukanganisa nharaunda asiwo anobvumira kudzoserwa kwezvinhu zvinokosha kubva munzizi yemarara. Uyezve, centrifuge dzinowanawo mashandisirwo muzvidzidzo zvesainzi yezvakatipoteredza, uko dzinoshandiswa kuongorora kusvibiswa kuri mumvura, ivhu, kana mhepo.

Mhando dzeCentrifuges uye Mashandiro Adzo

Mhando dzakasiyana dzema "centrifuge" dziripo, imwe neimwe yakagadzirirwa kuenderana nezvinodiwa zvekuparadzanisa. Mhando dzakajairika dzinosanganisira sedimentation centrifuge, filtration centrifuge, uye density gradient centrifuge.

Ma "sedimentation centrifuges" anozivikanwa nekutenderera kwawo nekukurumidza, anowanzo shandiswa mukuita apo zvidimbu zvidiki zvinogara zvichienderana nehukuru hwazvo uye huwandu hwazvo. Nekugadzirisa kumhanya kwekutenderera nenguva, zvidimbu zvidiki zvinogona kuparadzaniswa kubva kune zvikuru zvinobudirira.

Ma "centrifuge" ekusefa, kune rumwe rutivi, anoshandisa "filter medium" kubata zvidimbu uye kuzviparadzanisa nechikamu chemvura. Rudzi urwu rwe "centrifuge" runonyanya kubatsira mumabasa apo kuchena kwakakosha, sezvo zvichibvumira kudzora kwakanyatsojeka kwekuparadzaniswa kwehukuru hwezvidimbu.

Ma "density gradient centrifuges" anoshandisa simba re "centrifugal" pamwe chete ne "density gradient medium" kupatsanura zvinhu zvine "densities" dzakafanana. Iyi nzira inoshandiswa zvakanyanya mukupatsanura mapuroteni, mavhairasi, uye ma "organelles" emasero, uko kusiyana kudiki kwe "density" kunoita basa rakakosha.

Matekiniki Epamusoro uye Mafambiro Ari Kukura muCentrifugation

Kufambira mberi mu tekinoroji ye centrifugation kwakawedzera mashandisirwo ayo uye kwakawedzera kushanda zvakanaka kwemaitiro ekuparadzanisa. Imwe nzira inozivikanwa ndeye ultracentrifugation technique, iyo inoshandisa kumhanya kwakanyanya kuti iwane kuparadzanisa kwakanaka. Iyi technique yakawana mashandisirwo muminda yakaita seDNA sequencing uye nanoparticle analysis.

Mafambiro ari kubuda anotarisa pakuita kuti centrifuge ishande zvishoma uye otomatiki. Microcentrifuges, nehukuru hwayo hudiki uye kukurumidza kukurumidzira, iri kuwana mukurumbira mukushandiswa kwakasiyana-siyana mukuongorora nekutsvaga. Pamusoro pezvo, masisitimu e automated centrifugation anopa nyore uye kururama, zvichiita kuti kupatsanurwa kurambe kuripo pasina kupindira nemaoko.

Mhedziso

Kubva pamishonga kusvika pakugadzirisa marara, centrifugation yave nzira inoshandiswa zvakasiyana-siyana yekuparadzanisa ine mashandisirwo akawanda. Kugona kwayo kuparadzanisa zvinhu zvichienderana nehuwandu hwazvo kwakachinja maindasitiri akawanda, zvichibvumira kuwedzera kushanda zvakanaka, kuchena, uye kudzoreredzwa kwezviwanikwa. Nekufambira mberi kuri kuitika mu tekinoroji, matekiniki e centrifugation ari kuramba achichinja, zvichipa mhinduro dzekuparadzanisa dzakarurama uye dzinoshanda. Remangwana rine kufambira mberi kwakanaka, sezvo masayendisiti nemainjiniya vachiedza kuvandudza maitiro aya anokosha zvakanyanya.