Ko centrifuging inoita sei kupatsanura makemikari

Kugadzira centrifuging: Kuzarura Zvakavanzika zvekuparadzaniswa kwemakemikari

Nhanganyaya yeCentrifuging seMaitiro Ekuparadzanisa Makemikari

Kupinza zvinhu mu "centrifugation" inzira inokosha inoshandiswa zvakanyanya muzvikamu zvakasiyana-siyana zvesainzi, kunyanya mumakemikari ne biochemistry. Chinangwa chayo chikuru ndechekuparadzanisa zvinhu zvine huwandu hwakasiyana kana zvikamu nekushandisa simba re "centrifugal". Asi mubvunzo unoramba uripo ndewekuti: "centrifuging" inoita here kuti pave nekuparadzanisa makemikari? Chinyorwa chino chine chinangwa chekuongorora kuomarara kwe "centrifugation", tichiongorora mashandiro ayo, mashandisirwo ayo, uye kushanda kwayo senzira yekuparadzanisa makemikari.

Nheyo Iri Kuseri Kwekudzora Kutenderera

Kuti unzwisise kuti kupatsanurana kwemakemikari kunoita sei kuti kupatsanurwe kwemakemikari, zvakakosha kunzwisisa musimboti wayo. Nzira iyi inotsamira pakushandiswa kwesimba repakati, rinogadzirwa kana sample yatenderedzwa nekumhanya kwakanyanya. Zvinhu zviri mu sample zvinosangana nemunda wepakati, zvichikonzera kuti zvidimbu zvine density yakakura ku sediment nekukurumidza kupfuura zvine density yakaderera. Kupatsanurwa uku kunoitika nekuda kwekusiyana kwe sedimentation rates yezvikamu zvakasiyana mu sample.

Mhando dzeMatekiniki eCentrifugation

Kupinza zvinhu mu centrifugation kunogona kupatsanurwa muzvikamu zviviri: kugadzirira nekuongorora. Kupinza zvinhu mu centrifugation kunovavarira kupatsanura huwandu hwakawanda hwezvinhu kuti zvishandiswezve, ukuwo kuongorora zvinhu mu centrifugation kunotarisa pakuongorora hunhu hwezvikamu zviri mumushonga. Maitiro ese ari maviri anoshandisa nzira dzakasiyana, dzakadai se differential centrifugation, isopycnic centrifugation, uye velocity centrifugation, kuti pave nekupatsanurwa kwemakemikari kunoshanda.

Kuisa centrifuging munzvimbo dzeindasitiri

Kupinza zvinhu mu centrifugation kunoita basa guru mumaindasitiri akasiyana-siyana, kunyanya mumaindasitiri emishonga nemakemikari. Semuenzaniso, mukugadzira mishonga, kupinza zvinhu mu centrifugation kunoshandiswa kuparadzanisa tsvina yakasimba kubva mumvura, zvichiita kuti chigadzirwa chekupedzisira chive chakachena uye chine mhando yakanaka. Saizvozvowo, maitiro emakemikari anowanzoda kupatsanurwa kwemakemikari akasiyana, uye kupinza zvinhu mu centrifugation kunopa nzira inoshanda yekuparadzanisa zvinhu zvinodiwa.

Kuchengetedza nharaunda muKutsvagurudza kweBiomedical

Nzvimbo yezvekurapa inovimba zvakanyanya ne centrifugation kuti iparadzanise zvikamu zvakati, zvakaita semapuroteni, DNA, uye subcellular organelles. Nekushandisa masimba akasiyana-siyana e centrifugal uye ma reagents chaiwo, vaongorori vanogona kuparadzanisa ma biomolecule aya kubva mumisanganiswa yakaoma. Iyi nzira inovagonesa kudzidza zvikamu zvakati, zvichibatsira mukufambira mberi muminda yakaita se genetics, proteomics, uye cellular biology.

Kudzora nharaunda uye Kuongorora Zvakatipoteredza

Kuchenesa mvura kubva mumhepo (centrifugation) kunoshandiswawo mukuongorora nharaunda. Semuenzaniso, nzvimbo dzinochenesa mvura yakasviba dzinoshandisa ma "centrifuge" kuparadzanisa marara akaoma nemvura, zvichibatsira mukuchenesa. Uyezve, kusvibisa mvura kubva mumhepo (centrifugation) kunobvumira masayendisiti kuongorora marara netsvina zviri mumienzaniso yenharaunda, zvichipa ruzivo rwakakosha pamusoro pehutano hwezvakatipoteredza uye mwero wekusvibiswa.

Kuvandudzwa muTekinoroji yeCentrifugation

Kufambira mberi kwemazuva ano kwakachinja nzvimbo ye centrifugation, zvichiunza zvinhu zvitsva zvinowedzera kushanda kwayo uye kururama. Ma centrifuge anomhanyisa ane zvidzoreso zvakaoma anobvumira kugadziriswa kwakanyatsojeka kwekumhanya kwekutenderera, zvichiita kuti zvive nyore kupatsanurana kwemakemikari. Pamusoro pezvo, kugadzirwa kwe ultracentrifuges kunogonesa vaongorori kuparadzanisa nanoparticles nema macromolecules zvinobudirira.

Matambudziko uye Kuganhurirwa kweCentrifugation

Sezvinoita chero nzira yesainzi, centrifugation ine miganhu yayo. Dambudziko guru riri pakuona simba rakakodzera recentrifugal uye nguva inodiwa kuti pave nekupatsanurwa kwakakwana. Uyezve, mamwe ma biomolecules akapfava anogona kukuvadzwa kana kucheneswa panguva yekuita izvi. Zvakakosha kuti masayendisiti anzwisise miganhu iyi uye achinje nzira dzavo zvinoenderana neizvozvo kuti ave nechokwadi chekuti mhinduro dzakarurama.

Tariro dzeramangwana uye Zvitsva

Kugadzira centrifugation kuri kuramba kuchichinja nekukurumidza, nekutsvaga kuri kuenderera mberi kwakatarisana nekuvandudza kugona kwayo. Matekiniki matsva, akadai sekugadzira centrifugation yakavakirwa pa microfluidic, ari kugadzirwa kugadzirisa zvipingamupinyi zvenzira dzechinyakare. Pamusoro pezvo, kufambira mberi mukuita otomatiki uye kuongorora data kuri kuita kuti maitiro acho ave nyore, zvichivandudza kushanda zvakanaka uye kudzokororwa.

Mhedziso:

Kugadzira makemikari (centrifugation) pasina mubvunzo kunoita basa guru mukupatsanura makemikari munzvimbo dzakasiyana-siyana dzesainzi. Kubva pakushandiswa kwemaindasitiri kusvika kutsvagurudzo yemazuva ano, kugona kwayo kupatsanura zvikamu zvakasiyana zvichienderana nehuwandu hwezvinhu kwakakosha. Nekufambira mberi kuri kuenderera mberi uye kuvandudzwa kwehunyanzvi hunoenderana panguva imwe chete, kugadzira makemikari (centrifugation) kungangoramba kuri pamberi pekupatsanura makemikari, zvichitungamira kuwanikwa kutsva uye hunyanzvi mumakore anotevera.