He aha te tikanga o te decant i roto i te centrifugation
Te Whakakore i te Wai i roto i te Whakapūngao: He Tukanga Taketake mō te Wehewehe i ngā Ranunga
Kupu Whakataki:
He tikanga whānui te whakamahi i te centrifugation i roto i ngā taiwhanga pūtaiao me ngā taiwhanga ahumahi. Ko te takahuri i ngā tauira i ngā tere teitei hei whakaoho i te whakatō me te wehewehe i ngā wāhanga i runga i tō rātou mātotoru. Ko tētahi taahiraa nui o te tukanga centrifugation ko te tango i te wai, he mea nui tēnei ki te tango i te wāhanga e hiahiatia ana mai i te ranunga. Ko te whāinga o tēnei tuhinga he whakarato i tētahi māramatanga whānui mō te tikanga o te tango i te wai i roto i te centrifugation me tōna hiranga i roto i ngā tono maha.
1. Te Mātāpono o te Whakapūngao:
Hei mārama ki te tukanga whakawātea, me mārama ki te kaupapa matua o te centrifugation. Ka whakamahia e tēnei tikanga ngā rerekētanga o te mātotoru i waenga i ngā wāhanga o te ranunga. Ka tere ake te whakatōtō o ngā matūriki kiato ake mā te kaha hurihuri o te centrifuge, ka akiaki i a rātou kia tau ki raro i te ipu tauira.
2. Te Tukanga o te Whakatōtōtanga Pūwero:
I roto i te centrifugation, ka whakanohoia te ranunga ki roto i te ngongo rotor, i te pounamu centrifuge rānei, kātahi ka utaina ki roto i te centrifuge. I te hurihuri o te rotor i ngā tere teitei, ka pāngia te ranunga e ngā kaha centrifuge, ka heke ai ngā matūriki matotoru ki waho. Mā tēnei tukanga whakatōtō centrifuge ka whakarei ake i te wehenga o ngā matūriki, ā, ka āwhina i te whakahekenga i muri mai.
3. He aha te Whakakorenga?
Ko te tango i te wai i roto i te tauira he tukanga tango āta i te wai i roto i te tauira i muri i te whakatōtō. Ko te tikanga he ringihia te wai i roto i te tauira, me te waiho i te paru, i ngā wāhanga kāore e hiahiatia ana rānei. Ka taea te tango i te wai mā te ringa, mā te whakamahi rānei i ngā pūnaha tango aunoa, i runga i te tauine me ngā whakaritenga o te whakamātautau.
4. Te Hiranga o te Whakakorenga:
He mea nui te tango i ngā matū i roto i te centrifugation nā te mea ka taea te wehe me te kohikohi i ngā wāhanga motuhake, ka waiho ngā rauemi kāore e hiahiatia. He mea nui tana mahi i roto i ngā momo mahi, pēnei i:
- Hangarau Koiora: He mea nui te wetewete i ngā matū hei wehewehe i ngā pūmua, ngā waikawa karihi, me ngā para pūtau mai i ngā tauira koiora. Mā tēnei ka taea e ngā kairangahau te tiki i ngā wāhanga parakore e tika ana mō ngā tātaritanga o muri mai, pērā i te purenga pūmua, te raupapatanga DNA rānei.
- Tātari Taiao: I roto i ngā rangahau taiao, ka āwhina te whakawātea i te wai ki te wehewehe i ngā poke, ngā paru, ngā mea rauropi rānei mai i ngā tauira wai, oneone rānei. Ka āwhina tēnei tukanga i te tātari i ngā poke me te mārama ki ō rātou pānga ki ngā pūnaha rauropi.
- Te Hanga Rongoā: He mea nui te tango i ngā matū i roto i ngā tukanga hanga rongoā. Mā tēnei ka taea te wehewehe i ngā poke kāore e hiahiatia, kia mau ai te parakore me te whai huatanga o ngā hua rongoā. Hei tāpiri, ka āwhina te tango i ngā matū ki te whakaora me te purenga o ngā matū whai hua i te wā e hanga ana i te rongoā.
- Ahumahi Kai: Ka whakamahia te whakatōtōtanga pūwero hei wehe i ngā mea totoka mai i ngā wai i roto i te tukatuka kai. Hei tauira, ka taea te wehe i te kirīmi mai i te miraka, te wehe rānei i te wai hua mai i te penupenu, kia pai ake ai te kounga me te matotoru o te hua.
5. Ngā Tikanga Whakakorenga ā-Ringa me ngā Tikanga Whakakorenga Aunoa:
Ka taea te mahi ā-ringa i te wetewete, mā te whakamahi rānei i ngā pūnaha wetewete aunoa, i runga i te rahinga o te tauira, ngā whakaritenga tika, me te nui o te tukatuka.
- Te Whakakore ā-Ringa: I roto i ngā mahi iti, ka whakamahia te whakakore ā-ringa. Whai muri i te tukanga whakapūtā, ka ringihia mārire te wai mā te whakamahi i te pipette, kia kore ai e whakakorikoria te paru. Me tūpato tēnei tikanga kia kore ai e whakakorikoria te wehenga e hiahiatia ana.
- Te Whakakorenga Aunoa: Ko ngā tono nui ake, ko ngā tukanga rānei e hiahia ana kia tino tika ake, ka whakamahi i ngā pūnaha whakakorenga aunoa. Ka taea te whakauru i ēnei pūnaha ki ngā centrifuge, kia taea ai te wehewehenga whakahaeretia me te ōrite. Ka whakaitihia e te whakakorenga aunoa te rerekētanga o te kaiwhakahaere, ā, ka whakapiki ake i te pai o te mahi.
Whakatau:
He mea nui te tango i ngā matū i roto i te tukanga whakapūkara, e āhei ai ngā kairangahau me ngā ahumahi ki te tango i ngā wāhanga e hiahiatia ana mai i ngā ranunga me te whai hua. Mā te mārama ki ngā mātāpono me ngā tikanga o te tango matū, ka taea e ngā kaipūtaiao te whakapai ake i ngā tikanga whakapūkara kia tutuki ai ngā wehenga kounga teitei. Ka kitea te whakamahinga o tēnei tukanga taketake i roto i ngā momo mara, e whai wāhi ana ki ngā whanaketanga o te rangahau, te hanga, te tātari taiao, me te tukatuka kai.
.