He aha ngā āhuatanga e pā ana ki te whai huatanga o te wehenga o te pūwhakawhiti ngongo?
He tikanga whānui te whakamahi i te centrifugation i roto i ngā ahumahi maha mō te wehewehe i ngā matūriki o ngā matotoru rerekē i roto i te wai i runga i tō rātou kaha centrifugation. Ko ngā centrifugation ngongo, ina koa, e mōhiotia ana mō tō rātou tino pai o te wehewehenga nā runga i ō rātou hoahoa me ngā mātāpono whakahaere. Heoi, he maha ngā āhuatanga ka pā ki te pai o te wehenga o te centrifugation ngongo, ka pā ki te mahi whānui me te putanga o te tukanga.
Te Kaha Pūtahi
Ko te kaha whakatere te kaha matua e akiaki ana i te tukanga wehewehe i roto i te whakatere ngongo. Ka puta mai i te hurihanga tere o te rotor, ka neke ngā matūriki matotoru ki te pakitara o waho o te ngongo whakatere, ko ngā matūriki māmā ake e tata ana ki te pokapū. Ko te rahi o te kaha whakatere he rite tonu ki te tere o te hurihanga o te whakatere, ā, ko ngā tere teitei ake ka hua ake he pai ake te wehewehe. He mea nui anō hoki te radius o te rotor ki te whakatau i te kaha whakatere, me ngā radius nui ake ka whakaputa i ngā kaha kaha ake.
Ko te whai huatanga o te wehenga i roto i te pūwero ngongo he mea tino whakawhirinaki ki te hoahoa me te āhua o te rotor me te ngongo pūwero. Ko te roa me te whānui o te ngongo, me te koki piko, ka pā ki te tauira rere o te wai i roto i te pūwero me te ara o ngā matūriki. Mā te ngongo pūwero roa ake ka roa ake te wā pa i waenga i ngā matūriki me te mara pūwero, ka pai ake te wehenga. Ka pā hoki te whānui o te ngongo ki te tere taunga o ngā matūriki, ā, ko ngā whānui iti ake ka hua ake he kaha pūwero teitei ake, ā, ka pai ake te whai huatanga wehenga. Hei tāpiri, ka pā te koki piko o te ngongo pūwero ki te tauira rere me te wā noho o ngā matūriki, ā, ka pā ki te whai huatanga wehenga.
Rahi me te Matūriki
He mea nui anō hoki te rahi me te mātotoru o ngā matūriki e wehea ana ki te whakatau i te whai huatanga o te wehenga o te pūwero ngongo. He iti ake te tere taunga o ngā matūriki iti, ā, he ngāwari ake ki te nekehanga Brownian, ka ārai i tō rātou wehenga. I tētahi atu taha, ka tere ake te taunga o ngā matūriki nui nā te kaha o te kore e taea te aukati, ā, ka ngāwari ake te wehea e te kaha pūwero. Ka pā anō hoki te mātotoru o ngā matūriki ki ō rātou whanonga i roto i te pūwero, me ngā matūriki matotoru ake e neke tere atu ana ki te pakitara o waho i ngā matūriki māmā ake. He mea nui kia whakaarohia te tohatoha rahi me te mātotoru o ngā matūriki i roto i te whāngai whakauru hei whakapai ake i te whai huatanga o te wehenga o te pūwero.
Te Matatini o te Wai
Ka pā nui te matotoru o te wai e tukatukahia ana i roto i te pūwero ngongo ki te whai huatanga o te wehenga o te pūnaha. Ka taea e ngā matotoru teitei ake te hanga ātete atu ki te rere o te wai i roto i te ngongo pūwero, ka heke ai te whai huatanga o te wehenga. Ka taea e ngā wai matotoru te hanga paparanga rohe tata ki ngā pakitara o te ngongo, ka ārai i te nekehanga o ngā matūriki ki te pakitara o waho. Hei tāpiri, ka taea e ngā wai matotoru teitei te hua ake i te pikinga o te whakapaunga pūngao me te whakaputa wera, ka pā ki te mahi whānui o te pūwero. He mea nui kia ata whakahaere i te matotoru o te wai e tukatukahia ana kia nui ake ai te whai huatanga o te wehenga o te pūwero ngongo.
Tere Rere Whāngai
Ka pā nui te tere o te rere o te whāngai e tomo ana ki te centrifuge ki te pai o te wehenga o te pūnaha. Ka taea e ngā tere rere teitei ake te whakaiti i te wā noho o ngā matūriki i roto i te centrifuge, ka mutu, ka kore e oti te wehenga. Ka taea hoki e ngā tere rere nui te whakararuraru i roto i te ngongo centrifuge, ka whakararuraru i te tukanga wehenga me te whakaiti i te pai. I tētahi atu taha, ka roa ake pea te wā noho o ngā tere rere iti engari ka ārai pea, ka hoki whakamuri hoki te centrifuge. He mea nui kia arotauhia te tere rere o te whāngai kia tutuki ai te pai o te wehenga e hiahiatia ana, me te pupuri tonu i te pumau me te mahi a te centrifuge.
Te pāmahana whakahaere
Ka pā anō hoki te pāmahana whakahaere o te pūwero ngongo ki tōna whai huatanga wehewehe, inā koa ka whakamahia ngā rauemi tairongo-pāmahana. Ka pāngia te matotoru o te wai e ngā huringa pāmahana, te mātotoru o ngā matūriki, me te mahi whānui o te pūwero. Ka piki ake te matotoru o te wai i ngā pāmahana teitei, ka whakaiti i te whai huatanga wehewehe o te pūwero. Hei tāpiri, ka pāngia te pumau me te pono o ngā matūriki e ngā huringa pāmahana, ka pā ki tō rātou whanonga i roto i te mara pūwero. He mea nui kia aroturukihia, kia whakahaeretia hoki te pāmahana whakahaere o te pūwero kia pai ake ai tōna whai huatanga wehewehe me te whakarite i te kounga o ngā wāhanga kua wehea.
Hei whakamutunga, ko te whai huatanga o te wehenga o te pūwero ngongo ka pāngia e te huinga o ngā āhuatanga, tae atu ki te kaha pūwero, ngā tawhā hoahoa, ngā āhuatanga matūriki, ngā āhuatanga wai, te tere rere whāngai, me te pāmahana whakahaere. Mā te āta whakaaro me te whakahaere i ēnei āhuatanga, ka taea te arotau i te mahi a te pūwero me te whakatutuki i te whai huatanga wehenga mōrahi. Mā te mārama ki te pānga o ēnei āhuatanga ka āwhina i ngā kaiwhakahaere me ngā miihini ki te whakatau whai whakaaro hei whakapai ake i te whai huatanga me te whai huatanga whānui o te tukanga wehenga.
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