koj puas siv tau centrifugation los cais dej thiab xuab zeb
Kev Taw Qhia
Kev siv centrifugation yog ib txoj kev siv dav hauv ntau qhov chaw tshawb fawb uas cuam tshuam nrog kev sib cais cov khoom sib xyaw raws li lawv qhov ceev siv lub zog centrifugal. Hauv tsab xov xwm no, peb yuav tshawb nrhiav seb puas siv tau centrifugation los cais dej thiab xuab zeb. Peb yuav kawm txog cov ntsiab cai ntawm kev siv centrifugation, cov yam ntxwv ntawm dej thiab xuab zeb, thiab tham txog cov teeb meem thiab cov kev daws teeb meem rau kev sib cais ob yam khoom no. Yog li cia peb pib!
Nkag Siab Txog Kev Siv Centrifugation
Ua ntej yuav kawm txog kev sib cais dej thiab xuab zeb siv centrifugation, nws yog ib qho tseem ceeb kom nkag siab txog cov ntsiab cai yooj yim ntawm cov txheej txheem no. Centrifugation ua haujlwm los ntawm kev tig cov qauv ntawm qhov ceev ceev, uas tsim lub zog centrifugal uas thawb cov khoom me me kom cais lawv raws li lawv qhov ceev. Cov khoom me me hnyav dua feem ntau txav mus rau ntug sab nraud ntawm lub chamber tig, thaum cov khoom me me dua yuav nyob ze rau hauv nruab nrab.
Cov Khoom ntawm Dej thiab Xuab Zeb
Dej, ib yam khoom siv uas tseem ceeb thiab siv tau rau txhua yam, muaj qhov ceev li ntawm 1 gram ib cubic centimeter (g/cm³) ntawm qhov kub thiab txias hauv chav. Nws yog ib lub molecule polar, ua rau nws yog ib qho khoom siv zoo heev rau kev yaj thiab thauj ntau yam khoom. Ntawm qhov tod tes, xuab zeb feem ntau muaj silicon dioxide (SiO₂) thiab muaj qhov ceev ntau dua li dej, nruab nrab li ntawm 2.65 g/cm³.
Cov Teeb Meem Hauv Kev Sib Cais Dej Thiab Xuab Zeb
Qhov kev sib cais ntawm dej thiab xuab zeb ua rau muaj kev cov nyom loj heev vim lawv qhov sib txawv ntawm qhov ceev, vim tias cov xuab zeb me me dua li dej. Thaum sib xyaw, cov xuab zeb me me feem ntau yuav poob sai sai, ua rau cov txheej txheem lim dej lossis cov txheej txheem tso dej tsis zoo. Kev daws qhov teeb meem no yuav tsum tau siv centrifugation, ntawm lwm cov txheej txheem, kom ua tiav kev sib cais zoo.
Siv Centrifugation los cais dej thiab xuab zeb
Yuav kom cais dej thiab xuab zeb siv lub tshuab centrifugation, yuav tsum ua raws li ob peb kauj ruam tseem ceeb. Cia peb tham txog cov kauj ruam no kom meej hauv qab no.
1. Kev Npaj Qauv
Cov dej thiab xuab zeb sib tov yuav tsum tau npaj kom zoo ua ntej muab tso rau hauv lub tshuab centrifugation. Kauj ruam thawj zaug yog kom tau txais cov qauv sib xyaw zoo, xyuas kom meej tias cov xuab zeb tau faib sib npaug thoob plaws hauv dej.
2. Kev Xaiv Centrifuge
Kev xaiv lub centrifuge uas tsim nyog yog qhov tseem ceeb heev rau kev ua tiav kev sib cais zoo. Lub centrifuge yuav tsum muaj peev xwm tswj tau qhov ntim ntawm cov qauv thaum muab lub zog tig txaus los thawb cov xuab zeb mus rau ntawm phab ntsa ntawm lub raj centrifuge lossis lub thawv.
3. Cov Kev Ntsuas Centrifugation
Kev txiav txim siab txog cov yam ntxwv ntawm kev centrifugation uas tsim nyog yog qhov tseem ceeb heev. Cov yam ntxwv xws li qhov ceev ntawm centrifuge (kev tig ib feeb, lossis rpm) thiab lub sijhawm ua lub luag haujlwm tseem ceeb hauv kev cais dej thiab xuab zeb kom zoo. Tej zaum yuav tsum muaj kev sim ua kom zoo tshaj plaws rau txhua qhov sib tov.
4. Cov Txheej Txheem Centrifugation
Thaum twg tau teev cov kev ntsuas zoo tshaj plaws lawm, cov khoom sib tov yuav tsum tau muab sib xyaw ua ke rau hauv cov raj lossis cov thawv centrifuge uas tsim nyog. Muab cov raj tso rau ntawm lub kaum sab hauv ntawm lub rotor centrifuge tuaj yeem pab cais cov dej thiab xuab zeb kom zoo.
5. Kev Tshuaj Xyuas Cov Txiaj Ntsig
Tom qab centrifugation, tshuaj xyuas cov txheej sib cais hauv lub raj lossis lub thawv. Yog tias ua tiav, cov xuab zeb me me yuav raug nias kom nruj rau ntug sab nraud thaum cov dej tseem nyob ze rau nruab nrab. Ua tib zoo tshem cov txheej xuab zeb thiab sau cov dej seem, ua tiav cov txheej txheem sib cais.
Xaus lus
Kev siv lub tshuab centrifugation yeej yog ib txoj kev zoo rau kev cais dej thiab xuab zeb. Los ntawm kev siv lub zog centrifugal uas tsim thaum lub sijhawm tig ceev ceev, nws ua tau los cais ob yam khoom no raws li lawv qhov sib txawv ntawm qhov ceev. Txawm hais tias muaj teeb meem vim yog xuab zeb qhov ceev ceev, kev xaiv lub tshuab centrifuge zoo thiab kev ua kom zoo dua cov kev ntsuas centrifugation tuaj yeem kov yeej cov teeb meem no tau zoo. Kev sim ntxiv tuaj yeem ua rau muaj cov txheej txheem zoo dua los pab txhawb cov txheej txheem sib cais.
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