Lub centrifuge siv los cais cov khoom no:
Lub centrifuge siv los cais cov khoom no:
Kev Taw Qhia Txog Centrifugation thiab Nws Cov Ntawv Thov
Kev siv centrifugation yog ib txoj kev siv dav hauv ntau qhov chaw xws li biology, chemistry, thiab tshuaj. Nws cuam tshuam nrog kev sib cais cov tshuaj raws li lawv qhov ceev lossis qhov nrawm ntawm kev tso dej tawm. Centrifuges, cov tshuab muaj zog uas muaj lub rotor uas tig ntawm qhov ceev ceev, siv lub zog centrifugal los ua kom cov dej tawm sai dua. Tsab xov xwm no lub hom phiaj yog tshawb nrhiav ntau yam kev siv ntawm centrifugation thiab nws qhov tseem ceeb hauv kev tshawb fawb thiab cov txheej txheem kev lag luam.
Tshawb Nrhiav Lub Ntsiab Cai ntawm Centrifugation
Lub hauv paus ntsiab lus ntawm centrifugation tig ib puag ncig kev sib cuam tshuam ntawm lub zog centrifugal thiab qhov nrawm ntawm kev tso dej. Thaum muab cov khoom sib tov tso rau hauv lub centrifuge thiab lub rotor pib tig, lub zog centrifugal uas siv rau cov khoom me me uas muaj nyob hauv cov khoom sib tov ua rau lawv txav mus rau sab nraud. Qhov kev txav no ua rau muaj kev sib cais ntawm cov khoom nrog qhov sib txawv sib txawv, vim tias cov khoom hnyav dua tso dej sai dua rau hauv qab thaum cov khoom sib dua tsim cov txheej saum toj ntawm lawv.
Kev Sib Cais Cov Cheebtsam Ntshav Siv Centrifugation
Ib qho ntawm cov kev siv centrifugation feem ntau yog nyob rau hauv kev sib cais cov khoom ntawm cov ntshav. Thaum tag nrho cov ntshav raug centrifugation, nws faib ua peb txheej sib txawv: plasma, buffy txheej, thiab cov qe ntshav liab. Cov plasma, qhov kua ntawm cov ntshav, tseem nyob rau sab saum toj, thaum cov qe ntshav liab hnyav dua nyob rau hauv qab. Cov txheej buffy, uas muaj cov qe ntshav dawb thiab platelets, tsim ib txheej nyias nyias ntawm cov plasma thiab cov qe ntshav liab. Qhov kev sib cais no tso cai rau kev sib cais thiab kev tshuaj xyuas cov khoom ntawm cov ntshav tshwj xeeb rau lub hom phiaj kuaj mob lossis kev tshawb fawb kho mob.
Kev ntxuav cov protein los ntawm Ultracentrifugation
Ultracentrifugation, ib txoj kev siv centrifugation ceev ceev, feem ntau siv rau kev ntxuav cov protein. Los ntawm kev muab cov protein sib xyaw rau ultracentrifugation, ntau kauj ruam ntxuav tuaj yeem ua tiav. Ua ntej, kauj ruam centrifugation qeeb qeeb tshem tawm cov khib nyiab loj dua lossis cov khoom sib xyaw. Tom ntej no, high-speed ultracentrifugation cais cov protein soluble los ntawm cov khoom tsis yaj. Ntxiv mus, density gradient ultracentrifugation tuaj yeem siv los cais cov protein tshwj xeeb raws li lawv qhov ceev buoyant. Cov txheej txheem ntxuav kom meej no yog qhov tseem ceeb rau ntau yam kev siv, suav nrog kev piav qhia txog cov qauv protein, kev tshawb nrhiav tshuaj, thiab kev tshawb fawb biomedical.
Kev Siv Centrifugation Hauv Kev Ua Tshuaj
Kev siv centrifugation ua lub luag haujlwm tseem ceeb hauv kev lag luam tshuaj lom neeg rau ntau yam haujlwm sib cais. Nws pab cais cov khoom khov ntawm cov kua, rho tawm cov khoom muaj nqis, thiab ntxuav cov dej ntws. Piv txwv li, hauv kev tsim tshuaj, centrifugation yog siv los tshem tawm cov khoom tsis huv thiab cais cov khoom khov crystalline ntawm cov kua dej. Hauv kev lag luam roj thiab roj av, centrifuges cais cov roj thiab dej emulsions zoo, pab txhawb kev rov qab tau cov roj thiab ua kom muaj kev ua raws li ib puag ncig. Tsis tas li ntawd, centrifuges pom kev siv hauv cov chaw kho dej khib nyiab rau kev tshem cov sludge thiab hauv kev lag luam zaub mov rau kev ua kom cov kua txiv hmab txiv ntoo huv, kev sib cais cov rog, thiab kev ua cov mis nyuj.
Xaus lus, centrifugation yog ib txoj kev siv tau ntau yam rau kev sib cais hauv ntau qhov chaw tshawb fawb thiab kev lag luam. Nws lub peev xwm los sib cais cov tshuaj raws li qhov nrawm lossis qhov ceev ua rau muaj kev sib cais thiab kev ntxuav cov khoom tshwj xeeb. Txij li kev sib cais cov khoom ntshav thiab kev ntxuav cov protein rau kev tshawb fawb thiab kev siv kho mob mus rau kev pab txhawb kev ua tshuaj lom neeg thiab kev kho dej khib nyiab, centrifuges tau dhau los ua cov cuab yeej tseem ceeb hauv ntau qhov chaw. Kev txhim kho txuas ntxiv ntawm cov txheej txheem centrifugation yuav ua rau muaj kev nce qib thiab kev tsim kho tshiab yav tom ntej.
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