As we all know, serum separation is a tricky problem, so how to centrifuge serum separation? First of all, we analyze from the concept of serum:
blood serum: The fluid that separates the blood after coagulation.
The essence of blood coagulation is the conversion of soluble fibrinogen into insoluble fibrin in the plasma. Fibrous proteins are thin filaments that weave into webs of blood cells, forming gel-like clots. 30 minutes to 1 hour after blood clotting, the blood clot in the platelet systolic protein contraction, so that the clot back to harden, extrusion clear liquid, known as the serum. Serum differs from plasma in that it lacks fibrinogen and clotting factors involved in blood clotting, but has added a small amount of substances released by platelets during clotting.
So let me go back to the purpose of serum separation
Stand at room temperature (37 degrees) for more than half an hour, turn on the centrifuge, and centrifuge at 3500-4000rpm for 5-10 minutes. The serum was isolated. If measuring enzymes and other indicators easy to degrade, according to the need to put 4 degrees for more than half an hour, centrifuge. (The purpose of leaving it at 37 degrees for a while is to allow the blood to coagulate and release the serum, because when the blood coagulates, the fibrin contracts and the serum releases.)
Matters needing attention
1 The centrifugal speed is too large, easy to cause hemolysis
2 Animal blood, try not to stick to hair, easy to hemolysis.
Tips: After blood removal, do not put the container upright, flat or tilt, so that the serum can expand the precipitation area, conducive to serum precipitation.
To sum up, the key problem is to collect blood well, grasp the room temperature standing time, and then use a special centrifuge for serum separation, in order to avoid hemolysis.